The differentially DNA-methylated region responsible for expression of runt-related transcription factor 2

Runt-related transcription factor 2 (Runx2) is essential for osteogenesis. This study aimes at identification of the genomic region differentially methylated in DNA for regulation of Runx2 expression. In the proximal promoter of mouse Runx2, DNA methylation was frequent at the region further than 3...

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Bibliographic Details
Published in:Journal of Veterinary Medical Science 2017, Vol.79(2), pp.230-237
Main Authors: WAKITANI, Shoichi, YOKOI, Daigo, HIDAKA, Yuichi, NISHINO, Koichiro
Format: Article
Language:English
Subjects:
Anatomy
Animals
Bone marrow
Bone Marrow Cells - cytology
Bone Marrow Cells - metabolism
Cbfa-1 protein
Cell Differentiation
Cells, Cultured
Core Binding Factor Alpha 1 Subunit - genetics
Core Binding Factor Alpha 1 Subunit - metabolism
correlation analysis
Demethylation
Deoxyribonucleic acid
DNA
DNA Methylation
Dogs
Gene Expression Regulation
Male
Mesenchyme
Mice
Mice, Inbred C57BL
Organs
Osteoblastogenesis
Osteoblasts
Osteoblasts - cytology
Osteoblasts - metabolism
Osteogenesis
promoter
Promoter Regions, Genetic
Runx2
Species Specificity
Transcription factors
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Summary:Runt-related transcription factor 2 (Runx2) is essential for osteogenesis. This study aimes at identification of the genomic region differentially methylated in DNA for regulation of Runx2 expression. In the proximal promoter of mouse Runx2, DNA methylation was frequent at the region further than 3 kb relative to the transcription start site, in contrast to lower methylation status of the closer locus within 2 kb from the transcription start site. At the intermediate part, we identified a novel differentially methylated region in the Runx2 promoter region (Runx2-DMR): from −2.7 to −2.2 kb relative to the start site of Runx2 transcription in mice. In this region, the DNA methylation rate correlated negatively with Runx2 expression among mouse organs as well as among primary cultures of bone marrow from different dogs. Induction of mouse and dog mesenchymal-like cells into osteoblastic differentiation decreased the methylation rate of Runx2-DMR. Thus, in this study, we identified a novel genomic region in which DNA methylation status is related to Runx2 expression and detected demethylation of Runx2-DMR during osteoblastic differentiation in mouse and dog.
ISSN:0916-7250
1347-7439
DOI:10.1292/jvms.16-0321