Hsa_circ_0054633 in peripheral blood can be used as a diagnostic biomarker of pre-diabetes and type 2 diabetes mellitus

Aims The purpose of the current study was to investigate the characteristic expression of circular RNAs (circRNAs) in the peripheral blood of type 2 diabetes mellitus (T2DM) patients and their potential as diagnostic biomarkers for pre-diabetes and T2DM. Methods CircRNAs in the peripheral blood from...

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Bibliographic Details
Published in:Acta diabetologica 2017-03, Vol.54 (3), p.237-245
Main Authors: Zhao, Zhenzhou, Li, Xuejie, Jian, Dongdong, Hao, Peiyuan, Rao, Lixin, Li, Muwei
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Biomarkers
Biomarkers - blood
Case-Control Studies
Cohort Studies
Diabetes
Diabetes Mellitus, Type 2 - blood
Diabetes Mellitus, Type 2 - diagnosis
Diabetes Mellitus, Type 2 - genetics
Down-Regulation
Female
Humans
Internal Medicine
Male
Medical diagnosis
Medicine
Medicine & Public Health
Metabolic Diseases
Microarray Analysis
Middle Aged
Original
Original Article
Prediabetic State - blood
Prediabetic State - diagnosis
Prediabetic State - genetics
Prognosis
Ribonucleic acid
RNA
RNA - blood
Sensitivity and Specificity
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Summary:Aims The purpose of the current study was to investigate the characteristic expression of circular RNAs (circRNAs) in the peripheral blood of type 2 diabetes mellitus (T2DM) patients and their potential as diagnostic biomarkers for pre-diabetes and T2DM. Methods CircRNAs in the peripheral blood from six healthy individuals and six T2DM patients were collected for microarray analysis, and an independent cohort study consisting of 20 normal cases, 20 pre-diabetes patients and 20 T2DM patients was conducted to verify the five chosen circRNAs. We then tested hsa_circ_0054633 in a third cohort (control group, n  = 60; pre-diabetes group, n  = 63; and T2DM group, n  = 64) by quantitative real-time polymerase chain reaction (Q-PCR). Results In total, 489 circRNAs were discovered to be differentially expressed between the two groups, and of these, 78 were upregulated and 411 were downregulated in the T2DM group. Five circRNAs were then selected as candidate biomarkers and further verified in a second cohort. Hsa_circ_0054633 was found to have the largest area under the curve (AUC). The diagnostic capacity of hsa_circ_0054633 was tested in a third cohort. After introducing the risk factors of T2DM, the hsa_circ_0054633 AUCs for the diagnosis of pre-diabetes and T2DM slightly increased from 0.751 (95% confidence interval [0.666–0.835], P  
ISSN:0940-5429
1432-5233
DOI:10.1007/s00592-016-0943-0