Therapeutic effects of different doses of polyethylene glycosylated porcine glucagon-like peptide-2 on ulcerative colitis in male rats

• Published in: BMC gastroenterology 2017-03, Vol.17 (1), p.34-34, Article 34
• Main Authors: Qi, Ke-Ke, Lv, Jia-Jia, Wu, Jie, Xu, Zi-Wei
• Format: Article
• Language: English
• Subjects: Absorptivity; Animal models; Animals; Balancing; Body weight; Body Weight - drug effects; Chemical compounds; Claudin-1 - drug effects; Colitis, Ulcerative - chemically induced; Colitis, Ulcerative - drug therapy; Colon; Colon - drug effects; Cytokines - drug effects; Dextran; Dextran Sulfate; Disease Models, Animal; Dose-Response Relationship, Drug; Duodenum - drug effects; Elution; Gastroenterology; Glucagon; Glucagon-like peptide 2; Glucagon-Like Peptide 2 - administration & dosage; Hogs; Incidence; Inflammatory bowel disease; Interleukin 1; Interleukin 10; Interleukin 7; Intestinal Mucosa - drug effects; Intestine; Male; Peptides; Polyethylene; Polyethylene Glycols - administration & dosage; Rats; Rodents; Sodium sulfate; Studies; Tumor necrosis factor-α; Tumors; Ulcerative colitis; Villus; Weaning; Zonula occludens-1 protein; Zonula Occludens-1 Protein - drug effects; γ-Interferon
• ISSN: 1471-230X; 1471-230X
• DOI: 10.1186/s12876-017-0593-x

Polyethylene glycosylated (PEGylated) porcine glucagon-like peptide-2 (pGLP-2) considerably increases half-life and stability compared with the native pGLP-2, but the effective dose for intestinal damage is still unclear. This study aims to evaluate the available dose of polyethylene glycosylated porcine glucagon-like peptide-2 (PEG-pGLP-2), a modified, long-acting form of pGLP-2 in an experimental rat model of ulcerative colitis. Thirty-five male rats were randomly assigned into five groups: control, dextran sodium sulphate (DSS), DSS + PEG-pGLP-2(L), DSS + PEG-pGLP-2(M) and DSS + PEG-pGLP-2(H). Rats in control group received only water; other rats were fed with 5% (w/v) DSS and intraperitoneally administered with 12.5, 25 and 100 nmol/kg PEG-pGLP-2 daily for 6 days. Compared with the control treatment, DSS treatment significantly (p