Detection and genotyping of Toxoplasma gondii isolated from soil in Ahvaz, southwest of Iran

To detection and genotype of Toxoplasma gondii isolated from soil in Ahvaz, southwest of Iran. Between August 2011 and May 2012 at different sites located in the area of the Ahvaz city south west Iran. A total of 200 soil samples were taken from different points of the region. Oocysts were recovered...

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Bibliographic Details
Published in:Journal of parasitic diseases 2017-03, Vol.41 (1), p.202-205
Main Authors: Saki, J., Khademvatan, S., Yousefi, E., Tavalla, M., Abdizadeh, R.
Format: Article
Language:English
Subjects:
Amplified fragment length polymorphism
Enzymes
Genotyping
Health Promotion and Disease Prevention
Infectious Diseases
Medicine
Medicine & Public Health
Oocysts
Original
Original Article
Polymerase chain reaction
Protozoa
Soil pollution
Toxoplasmosis
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Summary:To detection and genotype of Toxoplasma gondii isolated from soil in Ahvaz, southwest of Iran. Between August 2011 and May 2012 at different sites located in the area of the Ahvaz city south west Iran. A total of 200 soil samples were taken from different points of the region. Oocysts were recovered using the flotation method. Then, PCR reactions targeting the GRA6 gene were performed for specific T. gondii detection. The positive samples were studied by RFLP (random amplified fragment length polymorphism) using MseI enzymes to confirm the parasite linage. Toxoplasma DNA was found in 18 samples. Among them, 12 samples were successfully genotyped as GRA6 type III and 6 as GRA6 Type II. This is the first investigation detecting and genotyping T. gondii oocyst in environmental soil samples of Ahvaz, South west of Iran. The results of this study indicated that soil contaminated with T. gondii oocysts especially in public park may play a role in the epidemiology of human toxoplasmosis in southwest of Iran.
ISSN:0971-7196
0975-0703
DOI:10.1007/s12639-016-0778-1