Novel Sequential Screening and Enhanced Production of Fibrinolytic Enzyme by Bacillus sp. IND12 Using Response Surface Methodology in Solid-State Fermentation

Fibrinolytic enzymes have wide applications in clinical and waste treatment. Bacterial isolates were screened for fibrinolytic enzyme producing ability by skimmed milk agar plate using bromocresol green dye, fibrin plate method, zymography analysis, and goat blood clot lysis. After these sequential...

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Bibliographic Details
Published in:BioMed research international 2017-01, Vol.2017 (2017), p.1-13
Main Authors: Kim, Young Ock, Valan Arasu, Mariadhas, Abdullah Al-Dhabi, Naif, Arun, Arumugaperumal, Prakash Vincent, Samuel Gnana, Rajendran, P., Vijayaraghavan, Ponnuswamy, Young Kwon, Oh
Format: Article
Language:English
Subjects:
Agricultural production
Animals
Bacillus
Bacillus (Bacteria)
Bacillus - enzymology
Bacillus - growth & development
Bacterial Proteins - biosynthesis
Biomedical research
Biosynthesis
Blood clots
Cattle
Enzymes
Fermentation
Fibrinolytic Agents - metabolism
Goats
Industrial wastes
Medical research
Medical wastes
Methods
Microbial enzymes
Peptide Hydrolases - biosynthesis
Physiological aspects
Proteins
Studies
Sucrose
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Summary:Fibrinolytic enzymes have wide applications in clinical and waste treatment. Bacterial isolates were screened for fibrinolytic enzyme producing ability by skimmed milk agar plate using bromocresol green dye, fibrin plate method, zymography analysis, and goat blood clot lysis. After these sequential screenings, Bacillus sp. IND12 was selected for fibrinolytic enzyme production. Bacillus sp. IND12 effectively used cow dung for its growth and enzyme production (687±6.5 U/g substrate). Further, the optimum bioprocess parameters were found out for maximum fibrinolytic enzyme production using cow dung as a low cost substrate under solid-state fermentation. Two-level full-factorial experiments revealed that moisture, pH, sucrose, peptone, and MgSO4 were the vital parameters with statistical significance (p
ISSN:2314-6133
2314-6141
DOI:10.1155/2017/3909657