Computational On-Chip Imaging of Nanoparticles and Biomolecules using Ultraviolet Light

Significant progress in characterization of nanoparticles and biomolecules was enabled by the development of advanced imaging equipment with extreme spatial-resolution and sensitivity. To perform some of these analyses outside of well-resourced laboratories, it is necessary to create robust and cost...

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Bibliographic Details
Published in:Scientific reports 2017-03, Vol.7 (1), p.44157-44157, Article 44157
Main Authors: Daloglu, Mustafa Ugur, Ray, Aniruddha, Gorocs, Zoltan, Xiong, Matthew, Malik, Ravinder, Bitan, Gal, McLeod, Euan, Ozcan, Aydogan
Format: Article
Language:English
Subjects:
631/1647/328
639/624/1107/328
639/624/1107/510
Amyotrophic lateral sclerosis
Environmental monitoring
Humanities and Social Sciences
Illumination
Laboratories
multidisciplinary
Nanoparticles
Nucleic acids
Quality control
Science
Spatial discrimination
Superoxide dismutase
Ultraviolet radiation
Water quality
Wavelength
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Summary:Significant progress in characterization of nanoparticles and biomolecules was enabled by the development of advanced imaging equipment with extreme spatial-resolution and sensitivity. To perform some of these analyses outside of well-resourced laboratories, it is necessary to create robust and cost-effective alternatives to existing high-end laboratory-bound imaging and sensing equipment. Towards this aim, we have designed a holographic on-chip microscope operating at an ultraviolet illumination wavelength (UV) of 266 nm. The increased forward scattering from nanoscale objects at this short wavelength has enabled us to detect individual sub-30 nm nanoparticles over a large field-of-view of >16 mm 2 using an on-chip imaging platform, where the sample is placed at ≤0.5 mm away from the active area of an opto-electronic sensor-array, without any lenses in between. The strong absorption of this UV wavelength by biomolecules including nucleic acids and proteins has further enabled high-contrast imaging of nanoscopic aggregates of biomolecules, e.g., of enzyme Cu/Zn-superoxide dismutase, abnormal aggregation of which is linked to amyotrophic lateral sclerosis (ALS) - a fatal neurodegenerative disease. This UV-based wide-field computational imaging platform could be valuable for numerous applications in biomedical sciences and environmental monitoring, including disease diagnostics, viral load measurements as well as air- and water-quality assessment.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep44157