Analysis of distinct long noncoding RNA transcriptional fingerprints in pancreatic ductal adenocarcinoma

• Published in: Cancer medicine (Malden, MA) MA), 2017-03, Vol.6 (3), p.673-680
• Main Authors: Yu, Xiang, Lin, Yang, Sui, Wu, Zou, Yanfen, Lv, Zhongchuan
• Format: Article
• Language: English
• Subjects: Adenocarcinoma; Biomarker; Cancer Biology; Cancer therapies; Carcinoma, Pancreatic Ductal - genetics; CASC9; Cell adhesion & migration; Cell growth; Cell Line, Tumor; Cell Movement; Cell Proliferation; Databases, Genetic; Datasets; DNA microarrays; DNA probes; Esophagus; Gastric cancer; Gene expression; Gene Expression Profiling - methods; Gene Expression Regulation, Neoplastic; Genomes; Humans; LINC00152; lncRNA; Metastasis; Oligonucleotide Array Sequence Analysis - methods; Original Research; Pancreas; Pancreatic cancer; Pancreatic Neoplasms - genetics; PDAC; Proteins; Ribonucleic acid; RNA; RNA, Long Noncoding - genetics; Roles; Software; Studies; Transcription; Tumorigenesis
• ISSN: 2045-7634; 2045-7634
• DOI: 10.1002/cam4.1027

Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive and lethal malignancies with the worst prognosis. Recent studies have demonstrated that long noncoding RNAs (lncRNAs) play critical roles in tumorigenesis and cancer progression. However, the expression pattern and roles of lncRNAs in the development of PDAC remain unknown. Herein, we globally analyzed the lncRNA expression profile in human PDAC and non‐tumor tissues using four independent public microarray datasets from Gene Expression Omnibus (GEO). The analysis of GEO datasets by repurposing microarray probes confirmed that hundreds of lncRNAs are differentially expressed in PDAC tissues compared with normal tissues. We selected four lncRNAs including LINC00152, CASC9, LINC00226 and F11‐AS1 for validation in PDAC cell lines and normal cells. Loss of function assays were performed to investigate the roles of LINC00152 and CASC9 in PDAC cell proliferation and invasion. Taken together, our findings demonstrate lncRNA expression alterations in PDAC and may provide new potential molecular markers for PDAC patient diagnosis and treatment. We globally analyzed the lncRNAs profiling in human PDAC and non‐tumor tissues using 4 independent public microarray datasets, and found hundreds of lncRNAs were differently expressed in PDAC tissues. Furthermore, we validated the expression and function role two lncRNA, CASC9 and LIC00152 in PDAC cells.