Adipocyte-specific deletion of mTOR inhibits adipose tissue development and causes insulin resistance in mice

• Published in: Diabetologia 2016-09, Vol.59 (9), p.1995-2004
• Main Authors: Shan, Tizhong, Zhang, Pengpeng, Jiang, Qinyang, Xiong, Yan, Wang, Yizhen, Kuang, Shihuan
• Format: Article
• Language: English
• Subjects: Ablation; Adipocytes; Adipocytes - cytology; Adipocytes - metabolism; Adipogenesis - genetics; Adipogenesis - physiology; Adipose Tissue, Brown - cytology; Adipose Tissue, Brown - metabolism; Adipose Tissue, White - cytology; Adipose Tissue, White - metabolism; Animal sciences; Animals; Body fat; Cell Differentiation - genetics; Cell Differentiation - physiology; Energy; Energy Metabolism - genetics; Energy Metabolism - physiology; Glucose; Human Physiology; Insulin resistance; Insulin Resistance - genetics; Insulin Resistance - physiology; Internal Medicine; Kinases; Medicine; Medicine & Public Health; Metabolic Diseases; Metabolic disorders; Mice; Mice, Knockout; Obesity; PPAR gamma - genetics; PPAR gamma - metabolism; Proteins; TOR Serine-Threonine Kinases - genetics; TOR Serine-Threonine Kinases - metabolism
• ISSN: 0012-186X; 1432-0428
• DOI: 10.1007/s00125-016-4006-4

Aims/hypothesis The in vivo role of mechanistic target of rapamycin (mTOR) in the development and function of adipose tissue, especially brown adipose tissue (BAT), is not well understood. Here, we aimed to assess the effect of mTOR (also known as Mtor ) knockout on adipose tissues and systemic energy metabolism. Methods We generated adipocyte-specific mTOR -knockout mice ( Adipoq-mTOR ) by crossing adiponectin -Cre ( Adipoq-Cre ) mice with mTOR flox/flox mice. The mice were then subjected to morphological, physiological (indirect calorimetry, glucose and insulin tolerance tests) and gene expression analyses to determine the role of mTOR in adipose tissues. Results We provide in vivo evidence that mTOR is essential for adipose tissue development and growth. Deletion of mTOR decreased the mass of both BAT and white adipose tissues (WAT) and induced browning of WAT. In addition, ablation of mTOR in adipose tissues caused insulin resistance and fatty liver in the Adipoq-mTOR mice. Furthermore, mTOR was required for adipocyte differentiation in vivo and activation of PPARγ ameliorated the differentiation deficiency of the mTOR -null adipocytes. Conclusions/interpretation Our findings demonstrate that mTOR is a critical regulator of adipogenesis and systemic energy metabolism. Our study provides key insights into the role of mTOR in adipose tissues; such knowledge may facilitate the development of novel strategies with which to treat obesity and related metabolic diseases.