Linking the Belowground Microbial Composition, Diversity and Activity to Soilborne Disease Suppression and Growth Promotion of Tomato Amended with Biochar

• Published in: Scientific reports 2017-03, Vol.7 (1), p.44382-44382, Article 44382
• Main Authors: Jaiswal, Amit K., Elad, Yigal, Paudel, Indira, Graber, Ellen R., Cytryn, Eddie, Frenkel, Omer
• Format: Article
• Language: English
• Subjects: 45/23; 631/326/171/1818; 631/326/2522; Bacteria - classification; Bacteria - drug effects; Bacteria - genetics; Bacteria - growth & development; Biological control; Carbon sequestration; Charcoal; Charcoal - pharmacology; Colonization; Community structure; Fertilizers; Fungi - classification; Fungi - drug effects; Fungi - genetics; Fungi - growth & development; Fusarium - drug effects; Fusarium - growth & development; Fusarium - pathogenicity; High-Throughput Nucleotide Sequencing; Humanities and Social Sciences; Microbial Consortia - drug effects; Microbial Consortia - genetics; Microorganisms; multidisciplinary; Plant diseases; Plant Diseases - prevention & control; Plant growth; Rhizosphere; RNA, Ribosomal, 16S - genetics; Rot; rRNA 16S; Science; Soil - chemistry; Soil Microbiology; Soil-borne diseases; Soils; Solanum lycopersicum - drug effects; Solanum lycopersicum - growth & development; Solanum lycopersicum - microbiology; Taxonomy; Tomatoes
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep44382

Biochar, in addition to sequestering carbon, ameliorating soil, and improving plant performance, can impact foliar and soilborne plant diseases. Nevertheless, the mechanisms associated with suppression of soilborne diseases and improved plant performances are not well understood. This study is designed to establish the relationships between biochar-induced changes in rhizosphere microbial community structure, taxonomic and functional diversity, and activity with soilborne disease suppression and enhanced plant performance in a comprehensive fashion. Biochar suppressed Fusarium crown and root-rot of tomato and simultaneously improved tomato plant growth and physiological parameters. Furthermore, biochar reduced Fusarium root colonization and survival in soil, and increased the culturable counts of several biocontrol and plant growth promoting microorganisms. Illumina sequencing analyses of 16S rRNA gene revealed substantial differences in rhizosphere bacterial taxonomical composition between biochar-amended and non-amended treatments. Moreover, biochar amendment caused a significant increase in microbial taxonomic and functional diversity, microbial activities and an overall shift in carbon-source utilization. High microbial taxonomic and functional diversity and activity in the rhizosphere has been previously associated with suppression of diseases caused by soilborne pathogens and with plant growth promotion, and may collectively explain the significant reduction of disease and improvement in plant performance observed in the presence of biochar.