Endothelial cells microparticle-associated protein disulfide isomerase promotes platelet activation in metabolic syndrome

• Published in: Oncotarget 2016-12, Vol.7 (50), p.83231-83240
• Main Authors: Fan, Guan-Qi, Qin, Ran-Ran, Li, Yi-Hui, Song, Dai-Jun, Chen, Tong-Shuai, Zhang, Wei, Zhong, Ming, Zhang, Yun, Xing, Yan-Qiu, Wang, Zhi-Hao
• Format: Article
• Language: English
• Subjects: Blood Platelets - drug effects; Blood Platelets - enzymology; Case-Control Studies; Cell-Derived Microparticles - drug effects; Cell-Derived Microparticles - enzymology; Cells, Cultured; Enzyme Activation; Enzyme Inhibitors - pharmacology; Human Umbilical Vein Endothelial Cells - drug effects; Human Umbilical Vein Endothelial Cells - enzymology; Humans; Metabolic Syndrome - blood; Metabolic Syndrome - enzymology; P-Selectin - blood; Platelet Activation - drug effects; Platelet Aggregation; Platelet Aggregation Inhibitors - pharmacology; Platelet Glycoprotein GPIIb-IIIa Complex - metabolism; Protein Disulfide-Isomerases - antagonists & inhibitors; Protein Disulfide-Isomerases - blood; Research Paper; Signal Transduction; Time Factors
• ISSN: 1949-2553; 1949-2553
• DOI: 10.18632/oncotarget.13081

Metabolic syndrome (MetS) is a common challenge in the world, and the platelet activation is enhanced in MetS patients. However, the fundamental mechanism that underlies platelet activation in MetS remains incompletely understood. Endothelial cells are damaged seriously in MetS patients, then they release more endothelial microparticles (EMPs). After all, whether the EMPs participate in platelet activation is still obscure. If they were, how did they work? We demonstrated that the levels of EMPs, PMPs (platelet derived microparticles) and microparticle-carried-PDI activity increased in MetS patients. IR endothelial cells released more EMPs, the EMP-PDI was more activated. EMPs can enhance the activation of CD62P, GPIIb/IIIa and platelet aggregation and this process can be partly inhibited by PDI inhibitor such as RL90 and rutin. Activated platelets stimulated by EMPs expressed more PDI on cytoplasm and released more PMPs. We obtained plasma from 23 MetS patients and 8 normal healthy controls. First we built insulin resistance (IR) model of human umbilical vein endothelial cells (HUVECs), and then we separated EMPs from HUVECs culture medium and used these EMPs to stimulate platelets. Levels of microparticles, P-selectin(CD62P), Glycoprotein IIb/IIIa (GPIIb/IIIa) were detected by flow cytometry and levels of EMPs were detected by enzyme-linked immunosorbent assay (ELISA). The protein disulfide isomerase (PDI) activity was detected by insulin transhydrogenase assay. Platelet aggregation was assessed by turbidimetry. EMPs can promote the activation of GPIIb/IIIa in platelets and platelet aggregation by the PDI which is carried on the surface of EMPs.