Extracellular matrix and α5β1 integrin signaling control the maintenance of bone formation capacity by human adipose-derived stromal cells

Stromal vascular fraction (SVF) cells of human adipose tissue have the capacity to generate osteogenic grafts with intrinsic vasculogenic properties. However, adipose-derived stromal/stem cells (ASC), even after minimal monolayer expansion, display poor osteogenic capacity in vivo . We investigated...

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Published in:Scientific reports 2017-03, Vol.7 (1), p.44398-44398, Article 44398
Main Authors: Di Maggio, Nunzia, Martella, Elisa, Frismantiene, Agne, Resink, Therese J., Schreiner, Simone, Lucarelli, Enrico, Jaquiery, Claude, Schaefer, Dirk J., Martin, Ivan, Scherberich, Arnaud
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Language:English
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Humanities and Social Sciences
multidisciplinary
Science
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Summary:Stromal vascular fraction (SVF) cells of human adipose tissue have the capacity to generate osteogenic grafts with intrinsic vasculogenic properties. However, adipose-derived stromal/stem cells (ASC), even after minimal monolayer expansion, display poor osteogenic capacity in vivo . We investigated whether ASC bone-forming capacity may be maintained by culture within a self-produced extracellular matrix (ECM) that recapitulates the native environment. SVF cells expanded without passaging up to 28 days (Unpass-ASC) deposited a fibronectin-rich extracellular matrix and displayed greater clonogenicity and differentiation potential in vitro compared to ASC expanded only for 6 days (P0-ASC) or for 28 days with regular passaging (Pass-ASC). When implanted subcutaneously, Unpass-ASC produced bone tissue similarly to SVF cells, in contrast to P0- and Pass-ASC, which mainly formed fibrous tissue. Interestingly, clonogenic progenitors from native SVF and Unpass-ASC expressed low levels of the fibronectin receptor α 5 integrin (CD49e), which was instead upregulated in P0- and Pass-ASC. Mechanistically, induced activation of α 5 β 1 integrin in Unpass-ASC led to a significant loss of bone formation in vivo . This study shows that ECM and regulation of α 5 β 1 -integrin signaling preserve ASC progenitor properties, including bone tissue-forming capacity, during in vitro expansion.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep44398