P300 promotes migration, invasion and epithelial-mesenchymal transition in a nasopharyngeal carcinoma cell line

A previous study demonstrated that p300 is overexpressed in nasopharyngeal carcinoma (NPC), and that its expression is an independent prognostic factor. The aim of the present study is to investigate the role of in human NPC development. A small hairpin (sh) RNA lentiviral expression vector targetin...

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Bibliographic Details
Published in:Oncology letters 2017-02, Vol.13 (2), p.763-769
Main Authors: Liao, Zhi-Wei, Zhao, Lei, Cai, Mu-Yan, Xi, Mian, He, Li-Ru, Yu, Fang, Zhou, Tong-Chong, Liu, Meng-Zhong
Format: Article
Language:English
Subjects:
Care and treatment
Cell adhesion & migration
Cell growth
Development and progression
Experiments
Gene expression
Genetic aspects
Health aspects
Liver cancer
Metastasis
Nasopharyngeal cancer
Oncology
Proteins
Radiation therapy
Studies
Throat cancer
Tumor proteins
Wound healing
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Summary:A previous study demonstrated that p300 is overexpressed in nasopharyngeal carcinoma (NPC), and that its expression is an independent prognostic factor. The aim of the present study is to investigate the role of in human NPC development. A small hairpin (sh) RNA lentiviral expression vector targeting the gene was constructed to suppress the expression of p300 in NPC cells. Knockdown of was verified by reverse transcription-quantitative polymerase chain reaction and western blotting. Wound-healing, invasion, immunofluorescence and immunoprecipitation assays were performed to assess the influence of p300 on nasopharyngeal tumorigenesis and metastasis . The expression of p300 was upregulated in NPC cell lines. After knockdown of , the migration and invasion ability of shp300 cells were significantly inhibited (P
ISSN:1792-1074
1792-1082
DOI:10.3892/ol.2016.5491