Effect of pesticides on microbial communities in container aquatic habitats

• Published in: Scientific reports 2017-03, Vol.7 (1), p.44565, Article 44565
• Main Authors: Muturi, Ephantus J., Donthu, Ravi Kiran, Fields, Christopher J., Moise, Imelda K., Kim, Chang-Hyun
• Format: Article
• Language: English
• Subjects: 45; 45/22; 631/326/2565/855; Agricultural land; Agriculture; Animals; Aquatic ecosystems; Aquatic habitats; Aquatic Organisms - drug effects; Aquatic Organisms - genetics; Aquatic Organisms - microbiology; Aquatic plants; Atrazine; Atrazine - toxicity; Carbaryl; Carbaryl - toxicity; Community structure; Containers; Culicidae - drug effects; Ecosystem; Genetic Variation - drug effects; Genetic Variation - genetics; Glycine - analogs & derivatives; Glycine - toxicity; Glyphosate; Herbicides; Herbicides - toxicity; Humanities and Social Sciences; Insecticides; Larva - drug effects; Macroinvertebrates; Malathion; Malathion - toxicity; Microbial activity; Microbiomes; Microcosms; multidisciplinary; Organic matter; Permethrin; Permethrin - toxicity; Pesticides; Pesticides - toxicity; RNA, Ribosomal, 16S - genetics; rRNA 16S; Science; Water Pollutants, Chemical - toxicity
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep44565

Container aquatic habitats support a specialized community of macroinvertebrates (e.g. mosquitoes) that feed on microbial communities associated with decaying organic matter. These aquatic habitats are often embedded within and around agricultural lands and are frequently exposed to pesticides. We used a microcosm approach to examine the single and combined effects of two herbicides (atrazine, glyphosate), and three insecticides (malathion, carbaryl, permethrin) on microbial communities of container aquatic habitats. MiSeq sequencing of the V4 region of both bacterial and archaeal 16S rRNA gene was used to characterize the microbial communities of indoor microcosms that were either exposed to each pesticide alone, a mix of herbicides, a mix of insecticides, or a mix of all five insecticides. Individual insecticides but not herbicides reduced the microbial diversity and richness and two insecticides, carbaryl and permethrin, also altered the microbial community structure. A mixture of herbicides had no effect on microbial diversity or structure but a mixture of insecticides or all five pesticides reduced microbial diversity and altered the community structure. These findings suggest that exposure of aquatic ecosystems to individual pesticides or their mixtures can disrupt aquatic microbial communities and there is need to decipher how these changes affect resident macroinvertebrate communities.