Beneficial effects of paeoniflorin on non-alcoholic fatty liver disease induced by high-fat diet in rats

Non-alcoholic fatty liver disease (NAFLD) is the most prevalent form of chronic liver diseases. This study sought to evaluate the insulin-sensitizing effect of paeoniflorin (PF) on high-fat diet-induced NAFLD and possible molecular mechanisms. Male Sprague Dawley rats were fed a high-fat diet (HFD)...

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Published in:Scientific reports 2017-03, Vol.7 (1), p.44819, Article 44819
Main Authors: Ma, Zhihong, Chu, Li, Liu, Hongying, Wang, Weijie, Li, Jieru, Yao, Wenzao, Yi, Jianfeng, Gao, Yue
Format: Article
Language:English
Subjects:
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AKT protein
Alanine
Alanine transaminase
Aspartate aminotransferase
Cholesterol
Diet
Fatty liver
High fat diet
Homeostasis
Humanities and Social Sciences
Insulin
Insulin resistance
Lipid metabolism
Lipids
Liver
Liver diseases
Molecular modelling
multidisciplinary
Oxidative stress
Rodents
Science
Signal transduction
Steatosis
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Summary:Non-alcoholic fatty liver disease (NAFLD) is the most prevalent form of chronic liver diseases. This study sought to evaluate the insulin-sensitizing effect of paeoniflorin (PF) on high-fat diet-induced NAFLD and possible molecular mechanisms. Male Sprague Dawley rats were fed a high-fat diet (HFD) for 10 weeks to establish the NAFLD model, and PF (20 mg/kg/d) was gavaged to the NAFLD rats for another four weeks. Our results demonstrated that HFD resulted in hepatocellular ballooning, micro-/macrovesicular steatosis, and oxidative stress in the liver, accompanied by increased serum total cholesterol (TC), triglyceride (TG), free fatty acid (FFA), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) levels and homeostasis model of insulin resistance (HOMA-IR) index. PF treatment improved the biochemical and histopathological changes in NAFLD rats. Moreover, we also found that PF could inhibit lipid ectopic deposition via regulating lipid metabolism (inhibiting lipid synthesis of cholesterol and de novo pathway), and exert insulin sensitizing effect by regulating the insulin signaling pathway IRS/Akt/GSK3β and anti-oxidation. The study findings suggest that PF has therapeutic potential against NAFLD and that it acts through multiple signaling pathways.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep44819