Macrophage production and activation are dependent on TRIM33

The tripartite motif (TRIM) family of proteins plays important roles in innate immunity and antimicrobial infection. None of these proteins has been shown to directly regulate transcription of genes in monocyte/macrophage except TRIM33 that we have recently shown to be a macrophage specific transcri...

Full description

Saved in:
Bibliographic Details
Published in:Oncotarget 2017-01, Vol.8 (3), p.5111-5122
Main Authors: Gallouet, Anne-Sophie, Ferri, Federica, Petit, Vanessa, Parcelier, Aude, Lewandowski, Daniel, Gault, Nathalie, Barroca, Vilma, Le Gras, Stéphanie, Soler, Eric, Grosveld, Frank, Davidson, Irwin, Romeo, Paul-Henri
Format: Article
Language:English
Subjects:
Animals
Biochemistry, Molecular Biology
Cells, Cultured
Chromatin - genetics
Chromatin - metabolism
Chromatin Immunoprecipitation
DNA - metabolism
Gene Regulatory Networks
Life Sciences
Lipopolysaccharides - pharmacology
Macrophage Activation
Macrophages - cytology
Macrophages - drug effects
Macrophages - immunology
Mice
Myeloid Cells - cytology
Myeloid Cells - immunology
RAW 264.7 Cells
Research Paper
Sequence Analysis, DNA
Transcription Factors - genetics
Transcription Factors - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The tripartite motif (TRIM) family of proteins plays important roles in innate immunity and antimicrobial infection. None of these proteins has been shown to directly regulate transcription of genes in monocyte/macrophage except TRIM33 that we have recently shown to be a macrophage specific transcriptional inhibitor of Ifnb1. Using ChIP-seq analyses, we now report that TRIM33 is bound to two fold more genes in immature than in mature myeloid cell lines. When located near the same genes, TRIM33 is bound to different sequences in the two cell lines suggesting a role of TRIM33 in both immature and mature myeloid cells. Accordingly, expression of TRIM33 in immature myeloid cells is necessary for efficient production of small peritoneal macrophages, monocytes and bone marrow derived macrophage (BMDM) and TRIM33 targets a subset of genes involved in the inflammatory response only in mature myeloid cells. Functionally, this targeting is associated with impaired repression of pathways regulating the late phases of lipopolysaccharide (LPS) activation of BMDM and a high sensitivity to LPS in vivo when the trim33 gene is inactivated in mature myeloid cells. These findings pinpoint TRIM33 as an important transcriptional actor of monocyte/macrophage mediated inflammation.
ISSN:1949-2553
1949-2553
DOI:10.18632/oncotarget.13872