Polarization sensitive optical coherence microscopy for brain imaging

Optical coherence tomography (OCT) and optical coherence microscopy (OCM) have demonstrated the ability to investigate cyto- and myelo-architecture in the brain. Polarization-sensitive OCT provides sensitivity to additional contrast mechanisms, specifically the birefringence of myelination and, ther...

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Bibliographic Details
Published in:Optics letters 2016-05, Vol.41 (10), p.2213-2216
Main Authors: Wang, Hui, Akkin, Taner, Magnain, Caroline, Wang, Ruopeng, Dubb, Jay, Kostis, William J, Yaseen, Mohammad A, Cramer, Avilash, Sakadžić, Sava, Boas, David
Format: Article
Language:English
Subjects:
Animals
Birefringence
Brain - diagnostic imaging
Mice
Microscopy, Polarization - methods
Neuroimaging
Tomography, Optical Coherence - methods
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Summary:Optical coherence tomography (OCT) and optical coherence microscopy (OCM) have demonstrated the ability to investigate cyto- and myelo-architecture in the brain. Polarization-sensitive OCT provides sensitivity to additional contrast mechanisms, specifically the birefringence of myelination and, therefore, is advantageous for investigating white matter fiber tracts. In this Letter, we developed a polarization-sensitive optical coherence microscope (PS-OCM) with a 3.5 μm axial and 1.3 μm transverse resolution to investigate fiber organization and orientation at a finer scale than previously demonstrated with PS-OCT. In a reconstructed mouse brain section, we showed that at the focal depths of 20-70 μm, the PS-OCM reliably identifies the neuronal fibers and quantifies the in-plane orientation.
ISSN:0146-9592
1539-4794
DOI:10.1364/OL.41.002213