Sets of serum exosomal microRNAs as candidate diagnostic biomarkers for Kawasaki disease

Although Kawasaki disease is the main cause of acquired heart disease in children, no diagnostic biomarkers are available. We aimed to identify candidate biomarkers for diagnosing Kawasaki disease using serum exosomal microRNAs (miRNAs). Using frozen serum samples from a biobank, high-throughput mic...

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Bibliographic Details
Published in:Scientific reports 2017-03, Vol.7 (1), p.44706-44706, Article 44706
Main Authors: Jia, Hong-Ling, Liu, Chao-Wu, Zhang, Li, Xu, Wei-Jun, Gao, Xue-Juan, Bai, Jun, Xu, Yu-Fen, Xu, Ming-Guo, Zhang, Gong
Format: Article
Language:English
Subjects:
631/337/384/331
692/53/2421
Aneurysms
Biomarkers
Biomarkers - blood
Cardiovascular diseases
Children
Coronary artery disease
Exosomes - genetics
Exosomes - ultrastructure
Gene Expression Profiling
Heart diseases
Humanities and Social Sciences
Humans
Kawasaki disease
MicroRNAs
MicroRNAs - blood
MicroRNAs - genetics
miRNA
Mucocutaneous lymph node syndrome
Mucocutaneous Lymph Node Syndrome - blood
Mucocutaneous Lymph Node Syndrome - diagnosis
Mucocutaneous Lymph Node Syndrome - genetics
multidisciplinary
Polymerase chain reaction
Reference Standards
Reproducibility of Results
Science
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Summary:Although Kawasaki disease is the main cause of acquired heart disease in children, no diagnostic biomarkers are available. We aimed to identify candidate biomarkers for diagnosing Kawasaki disease using serum exosomal microRNAs (miRNAs). Using frozen serum samples from a biobank, high-throughput microarray technologies, two-stage real-time quantitative PCR, and a self-referencing strategy for data normalization, we narrowed down the list of biomarker candidates to a set of 4 miRNAs. We further validated the diagnostic capabilities of the identified miRNAs (namely, C T (miR-1246)-C T (miR-4436b-5p) and C T (miR-197-3p)-C T (miR-671-5p)) in 79 samples from two hospitals. We found that this 4-miRNA set could distinguish KD patients from other febrile patients as well as from healthy individuals in a single pass, with a minimal rate of false positives and negatives. We thus propose, for the first time, that serum exosomal miRNAs represent candidate diagnostic biomarkers for Kawasaki disease. Additionally, we describe an effective strategy of screening for biomarkers of complex diseases even when little mechanistic knowledge is available.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep44706