5D imaging via light sheet microscopy reveals cell dynamics during the eye-antenna disc primordium formation in Drosophila

5D images of engrailed (en ) and eye gone (eyg ) gene expressions during the course of the eye-antenna disc primordium (EADP) formation of Drosophila embryos from embryonic stages 13 through 16 were recorded via light sheet microscopy and analyzed to reveal the cell dynamics involved in the developm...

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Bibliographic Details
Published in:Scientific reports 2017-03, Vol.7 (1), p.44945-44945, Article 44945
Main Authors: Huang, Yu Shan, Ku, Hui Yu, Tsai, Yun Chi, Chang, Chin Hao, Pao, Sih Hua, Sun, Y. Henry, Chiou, Arthur
Format: Article
Language:English
Subjects:
14/19
14/35
14/63
38/1
631/136/2086
639/624
64/24
Eye
Humanities and Social Sciences
Insects
Microscopy
multidisciplinary
Science
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Summary:5D images of engrailed (en ) and eye gone (eyg ) gene expressions during the course of the eye-antenna disc primordium (EADP) formation of Drosophila embryos from embryonic stages 13 through 16 were recorded via light sheet microscopy and analyzed to reveal the cell dynamics involved in the development of the EADP. Detailed analysis of the time-lapsed images revealed the process of EADP formation and its invagination trajectory, which involved an inversion of the EADP anterior-posterior axis relative to the body. Furthermore, analysis of the en- expression pattern in the EADP provided strong evidence that the EADP is derived from one of the en -expressing head segments.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep44945