The lactoferricin B-derived peptide, LfB17-34, induces melanogenesis in B16F10 cells

Lactoferricin B (LfcinB), a peptide of bovine lactoferrin (LfB), exhibits multiple biological functions, including antimicrobial, antiviral, antioxidant and immuno-modulatory activities. However, the role of LfcinB-related peptides in melanogenesis remains unclear. In this study, a set of five Lfcin...

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Bibliographic Details
Published in:International journal of molecular medicine 2017-03, Vol.39 (3), p.595-602
Main Authors: Huang, Hsiu-Chin, Lin, Hsuan, Huang, Min-Chuan
Format: Article
Language:English
Subjects:
Care and treatment
Cellular proteins
Development and progression
Enzymes
Gene expression
Genetic aspects
Health aspects
Kinases
Lactoferrins
Mammals
Melanoma
Peptides
Phosphorylation
Pigmentation disorders
Proteins
Studies
Vitiligo
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Summary:Lactoferricin B (LfcinB), a peptide of bovine lactoferrin (LfB), exhibits multiple biological functions, including antimicrobial, antiviral, antioxidant and immuno-modulatory activities. However, the role of LfcinB-related peptides in melanogenesis remains unclear. In this study, a set of five LfcinB-related peptides was examined. We found that LfB17-34, an 18-mer LfcinB-derived peptide, increased melanogenesis in B16F10 melanoma cells without significantly affecting cell viability. LfB17-34 increased in vitro tyrosinase activity and melanin content in a dose-dependent manner. The results of RT-qPCR and western blot analyses showed that LfB17-34 increased the mRNA and protein expression of tyrosinase and tyrosinase-related protein 1 (Trp1). Moreover, LfB17-34 inhibited the phosphorylation of MAPK/Erk, but not p38 and Akt, and constitutively active MEK was able to reverse the LfB17-34-enhanced pigmentation, melanin content, and tyrosinase activity, suggesting a role of Erk signaling in the process of LfB17-34-mediated pigmentation. Taken together, these results suggest that LfB17-34 induces melanogenesis in B16F10 cells primarily through increased tyrosinase expression and activity and that LfB17-34 could be further developed for the treatment of hypopigmentation disorders.
ISSN:1107-3756
1791-244X
DOI:10.3892/ijmm.2017.2884