MicroRNA-7 functions as a tumor-suppressor gene by regulating ILF2 in pancreatic carcinoma

Interleukin enhancer binding factor 2 (ILF2) has been found to be markedly upregulated in pancreatic carcinoma and is involved in the pathogenesis of pancreatic carcinoma. Thus, ILF2 may be a potential target for therapy. Yet, the regulatory mechanisms of ILF2 in pancreatic carcinoma remain largely...

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Bibliographic Details
Published in:International journal of molecular medicine 2017-04, Vol.39 (4), p.900-906
Main Authors: Bi, Yiliang, Shen, Wei, Min, Min, Liu, Yan
Format: Article
Language:English
Subjects:
Care and treatment
Cell growth
Cytokines
Development and progression
Gene expression
Genetic aspects
Health aspects
Infrared imaging systems
MicroRNA
Pancreatic cancer
Pathogenesis
Plasmids
Proteins
Transcription factors
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Summary:Interleukin enhancer binding factor 2 (ILF2) has been found to be markedly upregulated in pancreatic carcinoma and is involved in the pathogenesis of pancreatic carcinoma. Thus, ILF2 may be a potential target for therapy. Yet, the regulatory mechanisms of ILF2 in pancreatic carcinoma remain largely elusive. In the present study, we demonstrated that ILF2 functioned as an oncogene and regulated epithelial-mesenchymal transition (EMT)-associated genes in pancreatic carcinoma PANC-1 cells. MicroRNA-7 (miR-7) suppressed ILF2 mRNA expression and the protein level in PANC-1 cells. Contrary to ILF2, miRNA-7 functioned as a tumor-suppressor gene and negatively regulated EMT-associated genes in the PANC-1 cells. Curcumin, a polyphenol natural product isolated from the rhizome of the plant Curcuma longa, has emerged as a promising anticancer therapeutic agent. We found that treatment with curcumin increased miR-7 expression and suppressed ILF2 protein in the PANC-1 cells. Thus, we identified ILF2 as a new downstream target gene of curcumin. The results revealed that ILF2 is regulated by miR-7 and suggest that downregulation of miR-7 may be an important factor for the ILF2 overexpression in pancreatic carcinoma.
ISSN:1107-3756
1791-244X
DOI:10.3892/ijmm.2017.2894