Exploring the Association of Surface Plasmon Resonance with Recombinant MHC:Ig Hybrid Protein as a Tool for Detecting T Lymphocytes in Mice Infected with Leishmania (Leishmania) amazonensis

A surface plasmon resonance- (SPR-) based recognition method applying H-2 Ld:Ig/peptides complexes for ex vivo monitoring cellular immune responses during murine infection with Leishmania (Leishmania) amazonensis is described. Lymphocytes from lesion-draining popliteal lymph nodes were captured on a...

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Bibliographic Details
Published in:BioMed research international 2017-01, Vol.2017 (2017), p.1-9
Main Authors: Cavalcanti Júnior, Geraldo Barroso, Cysne-Finkelstein, Léa, Pereira, Bernardo Acácio Santini, Souza-Silva, Franklin, Silveira-Júnior, Lenilton Silva da, Alves, Carlos R.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antigens
Cysteine Proteases - chemistry
Cysteine Proteases - immunology
Disease Models, Animal
Flow cytometry
Health aspects
Humans
Immune system
Immunity, Cellular
Infections
Leishmania - immunology
Leishmania - pathogenicity
Leishmania amazonensis
Leishmaniasis
Leishmaniasis, Cutaneous - immunology
Leishmaniasis, Cutaneous - parasitology
Leishmaniasis, Cutaneous - pathology
Lymphatic system
Lymphocytes
Mice
Multiprotein Complexes - immunology
Multiprotein Complexes - isolation & purification
Parasites
Parasitic diseases
Peptides - immunology
Peptides - isolation & purification
Physiological aspects
Proteins
Recombinant proteins
Surface Plasmon Resonance
T-Lymphocytes - immunology
T-Lymphocytes - pathology
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Summary:A surface plasmon resonance- (SPR-) based recognition method applying H-2 Ld:Ig/peptides complexes for ex vivo monitoring cellular immune responses during murine infection with Leishmania (Leishmania) amazonensis is described. Lymphocytes from lesion-draining popliteal lymph nodes were captured on a carboxylated sensor chip surface previously functionalized with H-2 Ld:Ig (DimerX) protein bound to synthetic peptides derived from the COOH-terminal region of cysteine proteinase B of L. (L.) amazonensis. In computational analysis, these peptides presented values of kinetic constants favorable to form complexes with H-2 Ld at neutral pH, with a Gibbs free energy ΔG°
ISSN:2314-6133
2314-6141
DOI:10.1155/2017/9089748