Immunization with Pneumocystis Cross-Reactive Antigen 1 (Pca1) Protects Mice against Pneumocystis Pneumonia and Generates Antibody to Pneumocystis jirovecii

pneumonia (PcP) is a life-threatening infection that affects immunocompromised individuals. Nearly half of all PcP cases occur in those prescribed effective chemoprophylaxis, suggesting that additional preventive methods are needed. To this end, we have identified a unique mouse surface protein, des...

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Bibliographic Details
Published in:Infection and immunity 2017-04, Vol.85 (4)
Main Authors: Tesini, Brenda L, Wright, Terry W, Malone, Jane E, Haidaris, Constantine G, Harber, Martha, Sant, Andrea J, Nayak, Jennifer L, Gigliotti, Francis
Format: Article
Language:English
Subjects:
Animals
Antibodies, Fungal - immunology
Antibody Specificity - immunology
Antigens, Fungal - administration & dosage
Antigens, Fungal - genetics
Antigens, Fungal - immunology
Cross Reactions
Fungal Proteins - administration & dosage
Fungal Proteins - genetics
Fungal Proteins - immunology
Fungal Vaccines - administration & dosage
Fungal Vaccines - immunology
Immunization
Mice
Microbial Immunity and Vaccines
Pneumocystis - genetics
Pneumocystis - immunology
Pneumocystis carinii
Pneumocystis carinii - genetics
Pneumocystis carinii - immunology
Pneumonia, Pneumocystis - immunology
Pneumonia, Pneumocystis - prevention & control
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Summary:pneumonia (PcP) is a life-threatening infection that affects immunocompromised individuals. Nearly half of all PcP cases occur in those prescribed effective chemoprophylaxis, suggesting that additional preventive methods are needed. To this end, we have identified a unique mouse surface protein, designated cross-reactive antigen 1 (Pca1), as a potential vaccine candidate. Mice were immunized with a recombinant fusion protein containing Pca1. Subsequently, CD4 T cells were depleted, and the mice were exposed to Pca1 immunization completely protected nearly all mice, similar to immunization with whole organisms. In contrast, all immunized negative-control mice developed PcP. Unexpectedly, Pca1 immunization generated cross-reactive antibody that recognized and Potential orthologs of Pca1 have been identified in Such cross-reactivity is rare, and our findings suggest that Pca1 is a conserved antigen and potential vaccine target. The evaluation of Pca1-elicited antibodies in the prevention of PcP in humans deserves further investigation.
ISSN:0019-9567
1098-5522
DOI:10.1128/IAI.00850-16