Influenza A virus hemagglutinin and neuraminidase act as novel motile machinery

Influenza A virus (IAV) membrane proteins hemagglutinin (HA) and neuraminidase (NA) are determinants of virus infectivity, transmissibility, pathogenicity, host specificity, and major antigenicity. HA binds to a virus receptor, a sialoglycoprotein or sialoglycolipid, on the host cell and mediates vi...

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Bibliographic Details
Published in:Scientific reports 2017-03, Vol.7 (1), p.45043-45043, Article 45043
Main Authors: Sakai, Tatsuya, Nishimura, Shin I., Naito, Tadasuke, Saito, Mineki
Format: Article
Language:English
Subjects:
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631/57/343/1361
Animals
Antigenicity
Cell Line, Tumor
Cell surface
Dogs
Exo-a-sialidase
HEK293 Cells
Hemagglutinin Glycoproteins, Influenza Virus - metabolism
Hemagglutinins
Host specificity
Humanities and Social Sciences
Humans
Infectivity
Influenza
Influenza A
Influenza A virus - metabolism
Influenza A virus - physiology
Madin Darby Canine Kidney Cells
Membrane proteins
Movement
multidisciplinary
Neuraminidase - metabolism
Pathogenicity
Pathogens
Receptors, Virus - metabolism
Science
Virus attachment
Virus Internalization
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Summary:Influenza A virus (IAV) membrane proteins hemagglutinin (HA) and neuraminidase (NA) are determinants of virus infectivity, transmissibility, pathogenicity, host specificity, and major antigenicity. HA binds to a virus receptor, a sialoglycoprotein or sialoglycolipid, on the host cell and mediates virus attachment to the cell surface. The hydrolytic enzyme NA cleaves sialic acid from viral receptors and accelerates the release of progeny virus from host cells. In this study, we identified a novel function of HA and NA as machinery for viral motility. HAs exchanged binding partner receptors iteratively, generating virus movement on a receptor-coated glass surface instead of a cell surface. The virus movement was also dependent on NA. Virus movement mediated by HA and NA resulted in a three to four-fold increase in virus internalisation by cultured cells. We concluded that cooperation of HA and NA moves IAV particles on a cell surface and enhances virus infection of host cells.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep45043