Differential dehydration effects on globular proteins and intrinsically disordered proteins during film formation

Globular proteins composed of different secondary structures and fold types were examined by synchrotron radiation circular dichroism spectroscopy to determine the effects of dehydration on their secondary structures. They exhibited only minor changes upon removal of bulk water during film formation...

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Bibliographic Details
Published in:Protein science 2017-04, Vol.26 (4), p.718-726
Main Authors: Yoneda, Juliana Sakamoto, Miles, Andew J., Araujo, Ana Paula Ulian, Wallace, B. A.
Format: Article
Language:English
Subjects:
Chemical bonds
Circular Dichroism
Dehydration
Desiccation
Dichroism
folding
Freeze drying
globular proteins
Hydrogen Bonding
Hydrogen bonds
intrinsically disordered proteins
Intrinsically Disordered Proteins - chemistry
Protein Folding
Proteins
Radiation
secondary structure
Spectroscopy
Synchrotron radiation
synchrotron radiation circular dichroism (SRCD) spectroscopy
unfolding
Water treatment
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Summary:Globular proteins composed of different secondary structures and fold types were examined by synchrotron radiation circular dichroism spectroscopy to determine the effects of dehydration on their secondary structures. They exhibited only minor changes upon removal of bulk water during film formation, contrary to previously reported studies of proteins dehydrated by lyophilization (where substantial loss of helical structure and gain in sheet structure was detected). This near lack of conformational change observed for globular proteins contrasts with intrinsically disordered proteins (IDPs) dried in the same manner: the IDPs, which have almost completely unordered structures in solution, exhibited increased amounts of regular (mostly helical) secondary structures when dehydrated, suggesting formation of new intra‐protein hydrogen bonds replacing solvent‐protein hydrogen bonds, in a process which may mimic interactions that occur when IDPs bind to partner molecules. This study has thus shown that the secondary structures of globular and intrinsically disordered proteins behave very differently upon dehydration, and that films are a potentially useful format for examining dehydrated soluble proteins and assessing IDPs structures.
ISSN:0961-8368
1469-896X
DOI:10.1002/pro.3118