A Novel Restraint Device for Injection of Galleria mellonella Larvae that Minimizes the Risk of Accidental Operator Needle Stick Injury

Larvae of the insect are increasingly being used for studying pathogenic microbes and their virulence mechanisms, and as a rapid model for screening novel antimicrobial agents. The larvae (waxworms) are most frequently infected by injection of pathogenic organisms into the haemocoel through the inse...

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Bibliographic Details
Published in:Frontiers in cellular and infection microbiology 2017-03, Vol.7, p.99-99
Main Authors: Dalton, James P, Uy, Benedict, Swift, Simon, Wiles, Siouxsie
Format: Article
Language:English
Subjects:
Animals
Anti-Infective Agents
Communicable Diseases - transmission
Disease Models, Animal
Finger Injuries - prevention & control
Injections - economics
Injections - instrumentation
Injections - methods
Larva - microbiology
Lepidoptera
Microbiology
Needlestick Injuries - prevention & control
Staphylococcus aureus - pathogenicity
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Summary:Larvae of the insect are increasingly being used for studying pathogenic microbes and their virulence mechanisms, and as a rapid model for screening novel antimicrobial agents. The larvae (waxworms) are most frequently infected by injection of pathogenic organisms into the haemocoel through the insect's prolegs. The mostly widely used method for restraining the waxworms for injection is by grasping them between the operator's fingers, which puts the operator at risk of needle stick injury, an important consideration when working with highly pathogenic and/or drug-resistant microorganisms. While use of a stab proof glove can reduce this risk of injury, it does so at the loss of manual dexterity and speed, resulting in a more labor-intensive, and cumbersome assay. We describe a simple cost effective device (the so-called " Grabber") for restraining waxworms for injection that keeps the operator's fingers clear of the needle thus reducing the risk of injury.
ISSN:2235-2988
2235-2988
DOI:10.3389/fcimb.2017.00099