Low-intensity pulsed ultrasound (LIPUS) prevents periprosthetic inflammatory loosening through FBXL2-TRAF6 ubiquitination pathway

Previous studies have shown that Low intensity pulsed ultrasound(LIPUS) prevents polyethylene-debris-induced periprosthetic loosening in vivo , but the details of the mechanism by which it does so remain unclear. In this article, we used polyethylene debris induced RAW 264.7 cells as the in vitro mo...

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Bibliographic Details
Published in:Scientific reports 2017-04, Vol.7 (1), p.45779-45779, Article 45779
Main Authors: Zhao, Xiang, Zhao, Gangsheng, Shi, Zhongli, Zhou, Chenhe, Chen, Yunlin, Hu, Bin, Yan, Shigui
Format: Article
Language:English
Subjects:
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Apoptosis
Bone implants
Cell proliferation
Cytokines
Humanities and Social Sciences
Inflammation
multidisciplinary
Polyethylene
Science
siRNA
TRAF6 protein
Ubiquitination
Ultrasonic imaging
Ultrasound
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Summary:Previous studies have shown that Low intensity pulsed ultrasound(LIPUS) prevents polyethylene-debris-induced periprosthetic loosening in vivo , but the details of the mechanism by which it does so remain unclear. In this article, we used polyethylene debris induced RAW 264.7 cells as the in vitro model, and tested the effect of LIPUS on this model. Changes in the level of inflammatory cytokines, cell proliferation, and apoptosis were assessed. Gene overexpression and siRNA technique were applied, and the levels of expression of FBXL2, TRAF6, ERK, and related inflammatory cytokines were also measured. Results indicated that FBXL2-mediated TRAF6 ubiquitination and degradation also plays an important role in aseptic periprosthetic loosening process, and LIPUS prevents such loosening by strengthening this pathway.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep45779