Comparative Study of Two ELISA Kits for Estimation of Antibodies to Hepatitis B Virus in Human Normal Immunoglobulin and Specific Immunoglobulin Intended for Intravenous or Intramuscular Use

Current study is conducted to evaluate method verification of two locally available kits manufactured by DSI & BIORAD for quantitative estimation of Hepatitis B virus antibodies in human normal immunoglobulin by using International standard of National Institute of Biological Standards and Contr...

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Bibliographic Details
Published in:Indian journal of clinical biochemistry 2017-06, Vol.32 (2), p.243-245
Main Authors: Prasad, J. P., Madhu, Y., Mandhan, Arushi, Garg, Payal, Prakash, Anu, Singh, Varsha, Ali, Daud, Kumar, Prdeep, Singh, Surinder, Soni, G. R., Singh, Varun
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Chemistry/Food Science
Enzyme-linked immunosorbent assay
Hepatitis
Hepatitis B
Hepatitis B virus
Immune system
Immunoglobulins
Life Sciences
Microbiology
Pathology
Plasma
Short Communication
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Summary:Current study is conducted to evaluate method verification of two locally available kits manufactured by DSI & BIORAD for quantitative estimation of Hepatitis B virus antibodies in human normal immunoglobulin by using International standard of National Institute of Biological Standards and Control. Four analyst perform five sets of test in duplicate analysing accuracy, precision, and limit of detection, sensitivity and specificity. Our results suggest that both DSI and BIORAD kits fulfil the validation criteria and are sensitive to detect up to 10 mIU concentration precisely and accurately. DSI kit is more precise at concentration 100 mIU and economically 4–5 times cheaper in local market; on the other hand, BIORAD kits provide larger detection range up to 1000 mIU.
ISSN:0970-1915
0974-0422
DOI:10.1007/s12291-016-0590-9