Peptide Fraction pOh2 Exerts Antiadipogenic Activity through Inhibition of C/EBP-α and PPAR-γ Expression in 3T3-L1 Adipocytes

Many studies have comprehensively examined the venom of Ophiophagus hannah snake. Its venom comprises different compounds exhibiting a wide range of pharmacological activities. In this investigation, four peptide fractions (PFs), ranging from 3 kDa to 10 kDa, isolated from the Vietnamese snake venom...

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Bibliographic Details
Published in:BioMed research international 2017-01, Vol.2017, p.4826595-9
Main Authors: Nguyen, Thi Tuyet Nhung, Ha, Thi Thu, Nguyen, Thi Hoa, Vu, Thi Hien, Truong, Nam Hai, Chu, Hoang Ha, Van Quyen, Dong
Format: Article
Language:English
Subjects:
3T3-L1 Cells
Adipocytes - drug effects
Adipocytes - metabolism
Adipogenesis - drug effects
Animals
CCAAT-Enhancer-Binding Protein-alpha - genetics
CCAAT-Enhancer-Binding Protein-alpha - metabolism
Chromatography, High Pressure Liquid
Lipid Metabolism - drug effects
Lipid Metabolism - genetics
Mice
Peptides - pharmacology
PPAR gamma - genetics
PPAR gamma - metabolism
RNA, Messenger - genetics
RNA, Messenger - metabolism
Snake Venoms - chemistry
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Summary:Many studies have comprehensively examined the venom of Ophiophagus hannah snake. Its venom comprises different compounds exhibiting a wide range of pharmacological activities. In this investigation, four peptide fractions (PFs), ranging from 3 kDa to 10 kDa, isolated from the Vietnamese snake venom of O. hannah were separated by HPLC and investigated for their inhibitory activity on adipogenesis in 3T3-L1 adipocytes. The most effective PF was then further purified, generating two peptides, pOh1 and pOh2. Upon investigation of these two peptides on 3T3-L1 adipocytes, it was revealed that, at 10 μg/mL, pOh2 was able to inhibit the lipid accumulation in 3T3-L1 adipocytes by up to 56%, without affecting cell viability. Furthermore, the pOh2 downregulated the gene expression of important transcription factors C/EBP-α and PPAR-γ. In addition, aP2 and GPDH adipocyte-specific markers were also significantly reduced compared to untreated differentiated cells. Taken together, pOh2 inhibited the expression of key transcription factors C/EBP-α and PPAR-γ and their target genes, aP2 and GPDH, thereby blocking the adipocyte differentiation. In conclusion, this novel class of peptide might have potential for in vivo antiobesity effects.
ISSN:2314-6133
2314-6141
DOI:10.1155/2017/4826595