Efficient generation of mice carrying homozygous double-floxp alleles using the Cas9-Avidin/Biotin-donor DNA system

The clustered, regularly interspaced, short palin- dromic repeats （CRISPR）/CRISPR-associated protein 9 （Casg） system is a versatile tool for genomic engineering in mammalian cells and organisms, enabling the intro- duction of site-specific genomic double-strand breaks （DSBs） [1]. The resulting DSBs...

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Bibliographic Details
Published in:Cell research 2017-04, Vol.27 (4), p.578-581
Main Authors: Ma, Ming, Zhuang, Fengfeng, Hu, Xiongbing, Wang, Bolun, Wen, Xian-Zi, Ji, Jia-Fu, Xi, Jianzhong Jeff
Format: Article
Language:English
Subjects:
Alleles
Animals
Avidin - metabolism
Base Sequence
Biomedical and Life Sciences
Biotin
Biotin - metabolism
Cell Biology
CRISPR-Associated Protein 9 - metabolism
DNA - metabolism
Genetic Techniques
Homozygote
Integrases - metabolism
Letter to the Editor
Life Sciences
Mice, Transgenic
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Summary:The clustered, regularly interspaced, short palin- dromic repeats （CRISPR）/CRISPR-associated protein 9 （Casg） system is a versatile tool for genomic engineering in mammalian cells and organisms, enabling the intro- duction of site-specific genomic double-strand breaks （DSBs） [1]. The resulting DSBs are repaired by at least two distinct and competitive mechanisms, nonhomolo- gous end-joining （NHEJ） and homology-directed repair （HDR）. The former results in insertions and deletions （indels）, whereas the latter leads to precise genetic modi- fication.
ISSN:1001-0602
1748-7838
DOI:10.1038/cr.2017.29