Screening for antibacterial and antibiofilm activity in Thai medicinal plant extracts against oral microorganisms

To evaluate the antibacterial activity of 12 ethanol extracts of Thai traditional herb against oral pathogens. The antibacterial activities were assessed by agar well diffusion, broth microdilution, and time-kill methods. Antibiofilm activity was investigated using a 3-[4,5-dimethyl-2-thiazolyl]-2,5...

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Bibliographic Details
Published in:Journal of Traditional and Complementary Medicine 2017-04, Vol.7 (2), p.172-177
Main Authors: Teanpaisan, Rawee, Kawsud, Pajaree, Pahumunto, Nuntiya, Puripattanavong, Jindaporn
Format: Article
Language:English
Subjects:
4-Chromanol
Antibacteria
Antibiofilm
Oral pathogens
Original
Piper betle
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Summary:To evaluate the antibacterial activity of 12 ethanol extracts of Thai traditional herb against oral pathogens. The antibacterial activities were assessed by agar well diffusion, broth microdilution, and time-kill methods. Antibiofilm activity was investigated using a 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2H-tetrazolium- bromide (MTT) assay. High performance liquid chromatography (HPLC), thin layer chromatography (TLC) fingerprinting, and TLC-bioautography were used to determine the active antibacterial compounds. Piper betle showed the best antibacterial activities against all tested strains in the minimal inhibitory concentration and minimal bactericidal concentration, ranged from 1.04-5.21 mg/mL and 2.08-8.33 mg/mL, respectively. Killing ability depended on time and concentrations of the extract. P. betle extract acts as a potent antibiofilm agent with dual actions, preventing and eradicating the biofilm. The major constituent of P. betle extract was 4-chromanol, which responded for antibacteria and antibiofilm against oral pathogens. It suggests that the ethanol P. betle leaves extract may be used for preventing oral diseases.
ISSN:2225-4110
2225-4110
DOI:10.1016/j.jtcme.2016.06.007