Quantitative analysis of mycosporine-like amino acids in marine algae by capillary electrophoresis with diode-array detection

[Display omitted] •The first CE method for mycosporine-like amino acids (MAAs) was developed and validated.•Five MAAs were determined in several marine macroalgae and a lichen.•Quantitative results were in agreement to published data.•HPLC specific problems (poor retention, long equilibration) are i...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2017-05, Vol.138, p.153-157
Main Authors: Hartmann, Anja, Murauer, Adele, Ganzera, Markus
Format: Article
Language:English
Subjects:
Algae
Amino Acids - chemistry
Antioxidants - chemistry
Aquatic Organisms
Capillary electrophoresis
Cyclohexanols - chemistry
Cyclohexanones - chemistry
Cyclohexylamines - chemistry
Electrophoresis, Capillary - methods
Glycine - analogs & derivatives
Glycine - chemistry
Mycosporine-like amino acids
Propylene Glycols - chemistry
Rhodophyta - chemistry
Seaweed - chemistry
Sunscreen compounds
Ultraviolet Rays
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Summary:[Display omitted] •The first CE method for mycosporine-like amino acids (MAAs) was developed and validated.•Five MAAs were determined in several marine macroalgae and a lichen.•Quantitative results were in agreement to published data.•HPLC specific problems (poor retention, long equilibration) are irrelevant if CE is used. Marine species have evolved a variety of physical or chemical strategies to diminish damage from elevated environmental ultraviolet radiation. Mycosporine-like amino acids, a group of widely distributed small water soluble compounds, are biologically relevant because of their photo-protective potential. In addition, presumed antioxidant and skin protective strategies raise the interest for possible medicinal and cosmetic applications. In this study the first CE method for the quantification of mycosporine-like amino acids in marine species is presented. A borate buffer system consisting of 30mM sodium tetraborate in water at a pH-value of 10.3 enabled the baseline separation of five MAAs, namely palythine, mycosporine-serinol, asterina-330, shinorine and porphyra-334, in 27min. Separation voltage, temperature and detection wavelength were 25kV, 25°C and 320nm, respectively. The optimized method was fully validated and applied for the quantitative determination of MAAs in the marine macroalgae Palmaria palmata, Porphyra umbilicalis, and Porphyra sp., as well as the lichen Lichina pygmaea.
ISSN:0731-7085
1873-264X
DOI:10.1016/j.jpba.2017.01.053