Evaluation of the time resolved fluorescence immunoassay (TRFIA) for the detection of varicella zoster virus (VZV) antibodies following vaccination of healthcare workers

Determination of varicella zoster virus (VZV) immunity in healthcare workers without a history of chickenpox is important for identifying those in need of vOka vaccination. Post immunisation, healthcare workers in the UK who work with high risk patients are tested for seroconversion. To assess the p...

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Bibliographic Details
Published in:Journal of virological methods 2011-03, Vol.172 (1), p.60-65
Main Authors: McDonald, S.L.R., Maple, P.A.C., Andrews, N., Brown, K.E., Ayres, K.L., Scott, F.T., Al Bassam, M., Gershon, A.A., Steinberg, S.P., Breuer, J.
Format: Article
Language:English
Subjects:
Adult
Antibodies
Antibodies, Viral - blood
Antibody
Antibody Affinity - immunology
antibody detection
antigens
Avidity
Biological and medical sciences
Chickenpox - prevention & control
Female
Fluorescence
Fluorescent Antibody to Membrane Antigen
Fluoroimmunoassay - standards
Fundamental and applied biological sciences. Psychology
health care workers
Health Personnel
Herpesvirus 3, Human - immunology
Human herpesvirus 3
Humans
Immunity
Immunization
Immunoassays
Male
Medical personnel
Microbiology
Middle Aged
normal values
patients
risk
Risk factors
Sensitivity and Specificity
Seroconversion
Techniques used in virology
Time-resolved fluorescence immunoassay
Vaccination
Vaccines
Varicella
Varicella zoster virus
Virology
vOka vaccine
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Summary:Determination of varicella zoster virus (VZV) immunity in healthcare workers without a history of chickenpox is important for identifying those in need of vOka vaccination. Post immunisation, healthcare workers in the UK who work with high risk patients are tested for seroconversion. To assess the performance of the time-resolved fluorescence immunoassay (TRFIA) for the detection of antibody in vaccinated as well as unvaccinated individuals, a cut-off was first calculated. VZV-IgG specific avidity and titres six weeks after the first dose of vaccine were used to identify subjects with pre-existing immunity among a cohort of 110 healthcare workers. Those with high avidity (≥60%) were considered to have previous immunity to VZV and those with low or equivocal avidity (
ISSN:0166-0934
1879-0984
DOI:10.1016/j.jviromet.2010.12.021