Using Quantitative Spectrometry to Understand the Influence of Genetics and Nutritional Perturbations On the Virulence Potential of Staphylococcus aureus

• Published in: Molecular & cellular proteomics 2017-04, Vol.16 (4), p.S15-S28
• Main Authors: Chapman, Jessica R., Balasubramanian, Divya, Tam, Kayan, Askenazi, Manor, Copin, Richard, Shopsin, Bo, Torres, Victor J., Ueberheide, Beatrix M.
• Format: Article
• Language: English
• Subjects: Bacteremia; Bacteria; Bacterial infections; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Correlation analysis; Cytotoxicity; Cytotoxins; Cytotoxins - genetics; Cytotoxins - metabolism; Endocarditis; Environmental conditions; Experimental design; Gene Expression Regulation, Bacterial; Genetics; Genotype; Growth conditions; Humans; Infections; Leukocytes (neutrophilic); Lysis; Mass spectrometry; Mass Spectrometry - methods; Mass spectroscopy; Mutants; Neutrophils - microbiology; Proteomics; Proteomics - methods; Relative abundance; Scientific imaging; Special Issue: Proteomics and Infectious Disease; Spectroscopy; Staphylococcal Infections - microbiology; Staphylococcus aureus; Staphylococcus aureus - genetics; Staphylococcus aureus - growth & development; Staphylococcus aureus - metabolism; Staphylococcus aureus - pathogenicity; Strains (organisms); Toxicity; Virulence; Virulence factors; Virulence Factors - genetics; Virulence Factors - metabolism
• ISSN: 1535-9476; 1535-9484
• DOI: 10.1074/mcp.O116.065581

Staphylococcus aureus (Sa) is the leading cause of a variety of bacterial infections ranging from superficial skin infections to invasive and life threatening diseases such as septic bacteremia, necrotizing pneumonia, and endocarditis. The success of Sa as a human pathogen is contributed to its ability to adapt to different environments by changing expression, production, or secretion of virulence factors. Although Sa immune evasion is well-studied, the regulation of virulence factors under different nutrient and growth conditions is still not well understood. Here, we used label-free quantitative mass spectrometry to quantify and compare the Sa exoproteins (i.e. exoproteomes) of master regulator mutants or established reference strains. Different environmental conditions were addressed by growing the bacteria in rich or minimal media at different phases of growth. We observed clear differences in the composition of the exoproteomes depending on the genetic background or growth conditions. The relative abundance of cytotoxins determined in our study correlated well with differences in cytotoxicity measured by lysis of human neutrophils. Our findings demonstrate that label-free quantitative mass spectrometry is a versatile tool for predicting the virulence of bacterial strains and highlights the importance of the experimental design for in vitro studies. Furthermore, the results indicate that label-free proteomics can be used to cluster isolates into groups with similar virulence properties, highlighting the power of label-free quantitative mass spectrometry to distinguish Sa strains.