Heartworm extract induces relaxation of isolated rat thoracic aorta

Isolated rat thoracic aortic strips undergoing noradrenaline-induced contraction were treated with an adult heartworm (HW) crude extract and then examined for isometric changes in tension. HW extract caused relaxation of endothelium-intact strips, but not endothelium-denuded strips. This effect was...

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Bibliographic Details
Published in:Journal of Veterinary Medical Science 2017, Vol.79(4), pp.740-744
Main Authors: KITOH, Katsuya, NAKAMURA, Hiroaki, KITAGAWA, Hitoshi, UNNO, Toshihiro, KOMORI, Seiichi, CHIOU, Shin-Pin, TAKASHIMA, Yasuhiro
Format: Article
Language:English
Subjects:
Animals
Aorta, Thoracic - drug effects
Dirofilaria immitis
Dirofilaria immitis - chemistry
Dogs
Endothelium, Vascular - drug effects
Female
heartworm extract
Internal Medicine
Male
Muscle Relaxation - drug effects
Muscle, Smooth, Vascular - drug effects
rat aorta
Rats
Rats, Wistar
relaxation
Tissue Culture Techniques
Tissue Extracts - pharmacology
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Summary:Isolated rat thoracic aortic strips undergoing noradrenaline-induced contraction were treated with an adult heartworm (HW) crude extract and then examined for isometric changes in tension. HW extract caused relaxation of endothelium-intact strips, but not endothelium-denuded strips. This effect was inhibited by treatment with NG-nitro-L-arginine methyl ester hydrochloride (L-NAME) and could be reversed by additional treatment with L-arginine. However, HW extract at a high concentration caused slight relaxation of endothelium-denuded strips, and relaxation persisted after L-NAME treatment in endothelium intact-strips. These data suggested that the relaxation induced by HW extract was mainly endothelium-dependent, nitric oxide-mediated, but in part, also endothelium-independent. In addition, a bioassay using isolated rat thoracic aortas may be a useful tool for investigating vasoactive substances in the HW extract.
ISSN:0916-7250
1347-7439
DOI:10.1292/jvms.16-0590