RNA interference-mediated gene silencing of cyclophilin A enhances the radiosensitivity of PAa human lung adenocarcinoma cells in vitro

Radiotherapy is currently the major therapeutic strategy for patients with lung cancer. However, radioresistance and various side effects continue to present challenging issues for this form of treatment. A recent study demonstrated that cyclophilin A (CyPA) was overexpressed in non-small cell lung...

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Bibliographic Details
Published in:Oncology letters 2017-03, Vol.13 (3), p.1619-1624
Main Authors: Jiang, Xin, Zhang, Qiao-Li, Tian, Ye-Hong, Huang, Jin-Chang, Ma, Guo-Lin
Format: Article
Language:English
Subjects:
Adenocarcinoma
Apoptosis
Cancer therapies
Care and treatment
Deoxyribonucleic acid
Development and progression
DNA
E coli
Gene therapy
Genetic aspects
Kinases
Lung cancer
Packaging
Patient outcomes
Plasmids
Protein expression
Proteins
Radiation therapy
Radiotherapy
Studies
Vectors (Biology)
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Summary:Radiotherapy is currently the major therapeutic strategy for patients with lung cancer. However, radioresistance and various side effects continue to present challenging issues for this form of treatment. A recent study demonstrated that cyclophilin A (CyPA) was overexpressed in non-small cell lung cancer and, therefore, presents a novel potential therapeutic target. In addition, gene-radiotherapy is a novel method for cancer treatment. Therefore, the objective of the present study was to investigate the potential effect of CyPA silencing on radiosensitivity in human lung adenocarcinoma . The stable CyPA-silencing lung adenocarcinoma (PAa) cell line was generated using lentivirus-mediated small hairpin RNAs. The knockdown of CyPA was determined using fluorescent microscopy and western blot analysis. Cells were irradiated using various doses of cobalt-60 (0, 2, 4, 6 and 8 Gy). The radiosensitizing effects were determined by a clonogenic survival assay. Apoptosis and cell cycle distribution were evaluated using flow cytometry. Silencing of CyPA significantly increased the apoptosis of PAa cells. In addition, the radiosensitivity of cells was markedly enhanced following CyPA silencing. Furthermore, silencing of CyPA, in combination with irradiation, induced G /M phase cell cycle arrest. Taken together, the data suggest that the silencing of CyPA, combined with radiation therapy, may increase the therapeutic efficacy of lung cancer treatment through regulation of the cell cycle and apoptosis-associated signaling pathways.
ISSN:1792-1074
1792-1082
DOI:10.3892/ol.2017.5667