Effect of trehalose on cryopreservation of pure peripheral blood stem cells

Stem cells are an important tool for the study of hematopoiesis. Despite developments in cryopreservation, post-thaw cell death remains a considerable problem. Cryopreservation protocol should limit cell damage due to freezing and ensure the recovery of the functional cell characteristics after thaw...

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Bibliographic Details
Published in:Biomedical reports 2017-03, Vol.6 (3), p.314-318
Main Authors: Martinetti, Daniela, Colarossi, Cristina, Buccheri, Simona, Denti, Gabriella, Memeo, Lorenzo, Vicari, Luisa
Format: Article
Language:English
Subjects:
Blood
Bone marrow
Cell death
Cell survival
Cloning
Cooling
Cryopreservation
Cryopreservation of organs, tissues, etc
Cryoprotectants
Cryoprotectors
Data processing
Flow cytometry
Freezing
Health aspects
Hematopoiesis
Hematopoietic stem cells
Magnetic fields
Melting
Peripheral blood
Recovery of function
Review boards
Stem cells
Studies
Thawing
Trehalose
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Summary:Stem cells are an important tool for the study of hematopoiesis. Despite developments in cryopreservation, post-thaw cell death remains a considerable problem. Cryopreservation protocol should limit cell damage due to freezing and ensure the recovery of the functional cell characteristics after thawing. Thus, the use of cryoprotectants is essential. In particular, the efficacy of trehalose has been reported for clinical purposes in blood stem cells. The aim of the current study was to establish an efficient method for biological research based on the use of trehalose, to cryopreserve pure peripheral blood stem cells. The efficacy of trehalose was assessed and the cell viability was evaluated. The data indicate that trehalose improves cell survival after thawing compared with the standard freezing procedure. These findings could suggest the potential for future trehalose application for research purposes in cell cryopreservation.
ISSN:2049-9434
2049-9442
DOI:10.3892/br.2017.859