Overexpression of SmeDEF Efflux Pump Decreases Aminoglycoside Resistance in Stenotrophomonas maltophilia

The SmeDEF pump of is negatively regulated by SmeT. In this study, strains KJΔT ( deletion mutant) and KJT-D (mutant with a defective SmeT-binding site) showed increased resistance to chloramphenicol/nalidixic acid/macrolides and susceptibility to aminoglycoside. Overexpression of the SmeDEF pump, i...

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Bibliographic Details
Published in:Antimicrobial agents and chemotherapy 2017-05, Vol.61 (5)
Main Authors: Huang, Yi-Wei, Lin, Cheng-Wen, Ning, Hsiao-Chen, Lin, Yi-Tsung, Chang, Yi-Chih, Yang, Tsuey-Ching
Format: Article
Language:English
Subjects:
Amikacin - pharmacology
Aminoglycosides
Aminoglycosides - pharmacology
Anti-Bacterial Agents
Anti-Bacterial Agents - pharmacology
Bacterial Proteins
Bacterial Proteins - biosynthesis
Bacterial Proteins - genetics
Chloramphenicol - pharmacology
Ciprofloxacin - pharmacology
Drug Resistance, Multiple, Bacterial - genetics
Erythromycin - pharmacology
Gentamicins - pharmacology
Kanamycin - pharmacology
Mechanisms of Resistance
Membrane Transport Proteins
Membrane Transport Proteins - biosynthesis
Membrane Transport Proteins - genetics
Stenotrophomonas maltophilia
Stenotrophomonas maltophilia - drug effects
Stenotrophomonas maltophilia - genetics
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Summary:The SmeDEF pump of is negatively regulated by SmeT. In this study, strains KJΔT ( deletion mutant) and KJT-D (mutant with a defective SmeT-binding site) showed increased resistance to chloramphenicol/nalidixic acid/macrolides and susceptibility to aminoglycoside. Overexpression of the SmeDEF pump, in either KJΔT or KJT-D , downregulated expression, which is responsible for the reduced aminoglycoside resistance. Furthermore, the SmeRySy two-component regulatory system was downregulated in response to SmeDEF overexpression, which supports its involvement in the regulatory circuit.
ISSN:0066-4804
1098-6596
DOI:10.1128/AAC.02685-16