Antioxidant, Anti-Inflammatory, and Analgesic Activities of Agrimonia eupatoria L. Infusion

Agrimony (Agrimonia eupatoria L.) (Ae) is used in traditional medicine to treat inflammatory and oxidative related diseases. Therefore, this study focuses on the anti-inflammatory and analgesic potential of Ae infusion (AeI). Phenolic compounds characterization was achieved by HPLC-PDA-ESI/MSn. To e...

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Published in:Evidence-based complementary and alternative medicine 2017-01, Vol.2017 (2017), p.1-13
Main Authors: Batista, M. Teresa, Figueiredo, I. Vitória, Cruz, Maria Teresa, Paranhos, António, Francisco, Vera, Liberal, Joana, Ferreira, J. Pinto, Costa, Gustavo, Santos, Telmo N., Castelo-Branco, M.
Format: Article
Language:English
Subjects:
Acetic acid
Acids
Analgesics
Analysis
Anti-inflammatory drugs
Antioxidants
Carrageenin
Chronic illnesses
Drug dosages
Flavonoids
Inflammation
Kidneys
Liver
Medical research
Medicine, Experimental
Organic acids
Oxidative stress
Pain
Pharmacy
Polyphenols
Signal transduction
Studies
Superoxide
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Summary:Agrimony (Agrimonia eupatoria L.) (Ae) is used in traditional medicine to treat inflammatory and oxidative related diseases. Therefore, this study focuses on the anti-inflammatory and analgesic potential of Ae infusion (AeI). Phenolic compounds characterization was achieved by HPLC-PDA-ESI/MSn. To evaluate antioxidant potential, 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide anion, hydroxyl radical, and SNAP assays were used. In vitro anti-inflammatory activity of AeI was investigated in LPS-stimulated macrophages by measuring the NO production. In vivo anti-inflammatory activity was validated using the mouse carrageenan-induced paw edema model. Peripheral and central analgesic potential was evaluated using the acetic acid-induced writhing and hot-plate tests, respectively, as well as the formalin assay to assess both activities. The safety profile was disclosed in vitro and in vivo, using MTT and hematoxylin assays, respectively. Vitexin, quercetin O-galloyl-hexoside, and kaempferol O-acetyl-hexosyl-rhamnoside were referred to in this species for the first time. AeI and mainly AePF (Ae polyphenolic fraction) showed a significant antiradical activity against all tested radicals. Both AeI and AePF decreased NO levels in vitro, AePF being more active than AeI. In vivo anti-inflammatory and analgesic activities were verified for both samples at concentrations devoid of toxicity. Agrimony infusion and, mainly, AePF are potential sources of antiradical and anti-inflammatory polyphenols.
ISSN:1741-427X
1741-4288
DOI:10.1155/2017/8309894