Effect of HSP27 and Cofilin in the injury of hypoxia/reoxygenation on hepatocyte membrane F-actin microfilaments

Hypoxia-reoxygenation (H/R) injury hepatocyte models were established to simulate the ischemia/reperfusion injury of transplanted organ. Through the study of the molecular mechanism of H/R on the F-actin damage of the liver cytomembrane, the mechanism of F-actin damage induced by ischemia and reperf...

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Bibliographic Details
Published in:Medicine (Baltimore) 2017-04, Vol.96 (16), p.e6658-e6658
Main Authors: Zhang, Yafei, Wang, Jiazhong, Ji, Hong, Lu, Hongwei, Lu, Le, Wang, Jinlong, Li, Yiming
Format: Article
Language:English
Subjects:
Actin Depolymerizing Factors - biosynthesis
Actins - biosynthesis
Animals
Apoptosis
Cell Enlargement
Cell Shape
Disease Models, Animal
Hepatocytes - drug effects
Hepatocytes - metabolism
Hepatocytes - ultrastructure
HSP27 Heat-Shock Proteins - biosynthesis
Humans
Observational Study
Rats
Reperfusion Injury - pathology
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger
Time Factors
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Summary:Hypoxia-reoxygenation (H/R) injury hepatocyte models were established to simulate the ischemia/reperfusion injury of transplanted organ. Through the study of the molecular mechanism of H/R on the F-actin damage of the liver cytomembrane, the mechanism of F-actin damage induced by ischemia and reperfusion was studied from the level of cell and molecule.The hypoxic environment of cells in vitro was simulated by chemical hypoxia agent CoCl2. Liver cells were detected by MTT, H/R group was subdivided into 3 subgroups: H/R 2, 4, and 6 h. Changes of cell shape and the growth state, apoptosis, ultrastructural changes, and the changes in F-actin microfilament content were observed. Heat shock protein 27 (HSP27), Cofilin, and F-actin gene and protein levels were determined by real-time polymerase chain reaction and western blot assay, respectively.Cells showed circular adherence growth under normal circumstances, while the spindle cells and shedding cells were significantly increased in H/R groups. Apoptosis cells in H/R group were increased significantly with the extension of hypoxia time. The number of endoplasmic reticulum was decreased significantly in the H/R group, the mitochondrion hydropic was degenerated and the glycogen was disappeared. The F-actin fibers in the H/R group were disordered, the morphology of the fibers was obviously decreased, and the fluorescence staining decreased obviously (P 
ISSN:0025-7974
1536-5964
DOI:10.1097/md.0000000000006658