Immunohistochemical characterization of urethane-induced lung tumors in CB6F1-Tg rasH2 mice

The purpose of this study was to define the histopathological characteristics of pulmonary neoplastic lesions, especially focusing on the origin of tumor cells, in urethane-treated Tg rasH2 mice. Bronchiolar-alveolar adenomas/adenocarcinomas were observed in the lungs from all of the urethane-treate...

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Bibliographic Details
Published in:Journal of Toxicologic Pathology 2017, Vol.30(2), pp.177-181
Main Authors: Sano, Tomoya, Watanabe, Takeshi, Ishimura, Yoshimasa, Anayama, Hisashi
Format: Article
Language:English
Subjects:
Alveoli
Ethyl carbamate
Lesions
Lung cancer
lung tumor
Lungs
Mice
Protein C
Rodents
Short Communication
Staining
Stem cell transplantation
Stem cells
Surfactant protein C
Tg rasH2 mice
Tumor cells
Tumors
urethane
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Summary:The purpose of this study was to define the histopathological characteristics of pulmonary neoplastic lesions, especially focusing on the origin of tumor cells, in urethane-treated Tg rasH2 mice. Bronchiolar-alveolar adenomas/adenocarcinomas were observed in the lungs from all of the urethane-treated animals. Immunohistochemically, these tumors showed an alveolar epithelial type II (AE2) cell phenotype demonstrating positive staining of surfactant protein C (SP-C). Cells expressing Clara cell 10 (CC10), a Clara cell marker, were also observed in a scattered manner in some tumors. Several SP-C and CC10 double-positive cells were observed in these tumors. Most of the urethane-induced pulmonary tumors were considered to have an AE2 cell phenotype, but the presence of SP-C and CC10 double positive cells in the tumors of Tg rasH2 mice suggests that some tumors arose from bronchioalveolar stem cells, which are known to express both SP-C and CC10.
ISSN:0914-9198
1881-915X
1347-7404
DOI:10.1293/tox.2016-0066