Endotoxin-induced autocrine ATP signaling inhibits neutrophil chemotaxis through enhancing myosin light chain phosphorylation

Although the neutrophil recruitment cascade during inflammation has been well described, the molecular players that halt neutrophil chemotaxis remain unclear. In this study, we found that lipopolysaccharide (LPS) was a potent stop signal for chemotactic neutrophil migration. Treatment with an antago...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2017-04, Vol.114 (17), p.4483-4488
Main Authors: Wang, Xu, Qin, Weiting, Xu, Xiaohan, Xiong, Yuyun, Zhang, Yisen, Zhang, Huafeng, Sun, Bingwei
Format: Article
Language:English
Subjects:
ATP
Autocrine signalling
Biological Sciences
Calcium
Calcium (intracellular)
Calcium influx
Cells
Chemotaxis
Connexin 43
Leukocyte migration
Leukocytes (neutrophilic)
Lipopolysaccharides
Myosin
Myosin-light-chain kinase
Pathogens
Phosphorylation
Players
Recruitment
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Summary:Although the neutrophil recruitment cascade during inflammation has been well described, the molecular players that halt neutrophil chemotaxis remain unclear. In this study, we found that lipopolysaccharide (LPS) was a potent stop signal for chemotactic neutrophil migration. Treatment with an antagonist of the ATP receptor (P2X1) in primary human neutrophils or knockout of the P2X1 receptor in neutrophil-like differentiated HL-60 (dHL-60) cells recovered neutrophil chemotaxis. Further observations showed that LPS-induced ATP release through connexin 43 (Cx43) hemichannels was responsible for the activation of the P2X1 receptor and the subsequent calcium influx. Increased intracellular calcium stopped neutrophil chemotaxis by activating myosin light chain (MLC) through the myosin light chain kinase (MLCK)-dependent pathway. Taken together, these data identify a previously unknown function of LPS-induced autocrine ATP signaling in inhibiting neutrophil chemotaxis by enhancing MLC phosphorylation, which provides important evidence that stoppage of neutrophil chemotaxis at infectious foci plays a key role in the defense against invading pathogens.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.1616752114