Phylogenetic typing and molecular detection of virulence factors of avian pathogenic Escherichia coli isolated from colibacillosis cases in Japanese quail

• Published in: Veterinary research forum 2017-01, Vol.8 (1), p.55-58
• Main Authors: Alizade, Hesam, Ghanbarpour, Reza, Jajarami, Maziar, Askari, Asma
• Format: Article
• Language: English
• Subjects: Adhesion; Aerobactin; Antigens; Bacteria; Cellulitis; Colibacillosis; Colleges & universities; Colonization; Deoxyribonucleic acid; DNA; E coli; Epithelium; Granuloma; Hemolysins; Infectious diseases; Intestine; Methods; Omphalitis; Original; Pathogenesis; Phylogenetics; Phylogeny; Pili; Poultry; Research centers; Tropical diseases; Urinary tract; Veterinary medicine; Virulence factors; Wildfowl; Yolk; Yolk sac
• ISSN: 2008-8140; 2322-3618

Colibacillosis Escherichia coli Japanese quail Phylogenetic group Virulence gene Abstract Colibacillosis caused by avian pathogenic Escherichia coli (APEC) is an economic threat to the poultry industry throughout the world. (ProQuest: ... denotes non-US-ASCII text omitted.) Introduction Avian pathogenic Escherichia coli (APEC) are responsible for a variety of extra-intestinal pathogens in poultry, including colibacillosis, yolk sac infection, cellulitis, coli-granuloma and omphalitis.1 Although Japanese quail (Coturnix coturnix japonica) are reported as resistant birds against many diseases, APEC strains have been isolated from colisepticemic poultry with colibacillosis.1 Colibacillosis is an economic threat to the poultry industry which is a worldwide infection.2 Virulence factors (VFs) of extra-intestinal pathogenic E. coli (ExPEC) participate in colonization, cellular invasion and consequently reduction of the host immunity responses.3 Some of the VFs such as fimbrial antigens (P, AC/I, F1A and Stg), iron acquisition systems (aerobactin) and toxins (cytolethal distending toxins and hemolysins) may enhance the ability of E. coli to grow in the tissues of broilers.4 Expression of adhesions such as S and P fimbriae are considered to be an essential factor in pathogenesis of these strains because of their fundamental abilities for the adherence to the epithelium cells of birds.5 Stordeur et al. reported fimbrial and afimbrial adhesion genes normally expressed in extra-intestinal and intestinal strains isolated from birds.6 P fimbriae are an important step for the beginning and expansion of human urinary tract infections, but their role in pathogenesis of avian isolates has not been elucidated, completely.5 The E. coli strains are genetically diverse, and strains...