Gene electrotransfer: from biophysical mechanisms to in vivo applications: Part 2 - In vivo developments and present clinical applications

Gene electrotransfer can be obtained not just on single cells in diluted suspension. For more than 10 years, this is a quasi routine strategy in tissue on the living animal and a few clinical trials have now been approved. New problems have been brought by the close contacts of cells in tissue both...

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Bibliographic Details
Published in:Biophysical reviews 2009-12, Vol.1 (4), p.185-191
Main Authors: Escoffre, Jean-Michel, Mauroy, Chloé, Portet, Thomas, Wasungu, Luc, Paganin-Gioanni, Aurelie, Golzio, Muriel, Teissié, Justin, Rols, Marie-Pierre
Format: Article
Language:English
Subjects:
Animal biology
Biochemistry
Biological and Medical Physics
Biological Techniques
Biomedical and Life Sciences
Biophysics
Biotechnology
Cell Biology
Life Sciences
Membrane Biology
Nanotechnology
Review
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Summary:Gene electrotransfer can be obtained not just on single cells in diluted suspension. For more than 10 years, this is a quasi routine strategy in tissue on the living animal and a few clinical trials have now been approved. New problems have been brought by the close contacts of cells in tissue both on the local field distribution and on the access of DNA to target cells. They need to be solved to provide a further improvement in the efficacy and safety of protein expression. There is a competition between gene transfer and cell destruction. Nevertheless, present results are indicative that electrotransfer is a promising approach for gene therapy. High level and long-lived expression of proteins can be obtained in muscles. This is used for a successful method of electrovaccination.
ISSN:1867-2450
1793-0480
1867-2469
DOI:10.1007/s12551-009-0019-2