Influence of carbohydrates on quantitative aspects of growth and embryo formation in wild carrot suspension cultures

• Published in: Plant physiology (Bethesda) 1977, Vol.59 (1), p.81-85
• Main Authors: Verma, D.C, Dougall, D.K
• Format: Article
• Language: English
• Subjects: Callus; Carbon; Cell growth; Cultured cells; Daucus carota; Embryogenesis; Embryos; Plant cells; Plant tissues; Plants; Sugars
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.59.1.81

Wild carrot (Daucus carota L.) cell suspensions were grown on a mineral salt medium supplemented with 10 mM myoinositol in the presence and absence of 2.25 μM 2,4-dichlorophenoxyacetic acid (2,4-D), and a variety of carbon sources. The data obtained on growth and embryo number in the absence of 2,4-D show that wild carrot suspensions were able to utilize sucrose, glucose, fructose, galactose, mannose, maltose, raffinose, or stachyose as a carbon source. A highly significant correlation between dry weight and embryo number was obtained regardless of the carbohydrate source suggesting the involvement of a common intermediate in the metabolism of the various sugars. In the presence of 2.25 μM 2,4-D, embryo formation was suppressed. Time course of dry weights obtained in the presence and absence of 2,4-D show that 2,4-D increased the growth rate of the tissue when glucose, fructose, mannose, or stachyose was used as the carbon source. The growth rates on other sugars remained unchanged under these conditions.