Subcellular localization of the cyanogenic glucoside of sorghum by autoradiography

The use of microautoradiography at the electron microscopic level indicates that the vacuole is the site of accumulation of the cyanogenic glucoside of Sorghum bicolor. When a specific biosynthetic precursor of dhurrin, p-hydroxy[3,5-3H]phenylacetaldoxime, was used, 90% of the tritium label was reco...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1977-04, Vol.59 (4), p.647-652
Main Authors: Saunders, James A., Conn, Eric E., Lin, Chin Ho, Stocking, C. Ralph
Format: Article
Language:English
Subjects:
Biosynthesis
Dehydration
Glucosides
Oximes
Plant tissues
Plants
Seedlings
Silver
Sorghum
Vacuoles
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Summary:The use of microautoradiography at the electron microscopic level indicates that the vacuole is the site of accumulation of the cyanogenic glucoside of Sorghum bicolor. When a specific biosynthetic precursor of dhurrin, p-hydroxy[3,5-3H]phenylacetaldoxime, was used, 90% of the tritium label was recovered in the vacuoles of tissue prepared for microautoradiography. L-[3,5-3H]Tyrosine and D-[1-3H(N)]glucose, non-specific precursors of dhurrin, of differing solubilities and biosynthetic capacity, were also fed to the shoots. The data obtained from these controls indicated that the high recovery of label in the vacuole of aldoxime-fed shoots was not indicative simply of the size of the vacuole, nor was it a result of movement of labeled compounds during preparation of the tissue for electron microscopy. The problem of movement of these labeled compounds during dehydration of tissue was dramatically reduced by chemical dehydration in 2,2-dimethoxypropane in less than 30 minutes rather than with routine dehydration in acetone or alcohol series for 24 hours.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.59.4.647