Expression profiles and function analysis of microRNAs in postovulatory aging mouse oocytes

In this study, microRNA (miRNA) profiles in postovulatory aging mouse oocytes were analyzed by microarray screening and RT-qPCR. Hierarchical cluster analysis on the microarray data and KEGG pathway enrichment analysis on the mRNAs targeted by differentially expressed (DE) miRNAs between two adjacen...

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Published in:Aging (Albany, NY.) NY.), 2017-04, Vol.9 (4), p.1186-1201
Main Authors: Wang, Tian-Yang, Zhang, Jie, Zhu, Jiang, Lian, Hua-Yu, Yuan, Hong-Jie, Gao, Min, Luo, Ming-Jiu, Tan, Jing-He
Format: Article
Language:English
Subjects:
Animals
Calcium - metabolism
Caspase 3 - biosynthesis
Caspase 3 - genetics
Computational Biology
Female
Gene Expression Profiling
Gene Expression Regulation, Developmental - genetics
Mice
Microarray Analysis
MicroRNAs - antagonists & inhibitors
MicroRNAs - biosynthesis
MicroRNAs - genetics
Oocytes - metabolism
Oocytes - physiology
Ovulation
Polymerase Chain Reaction
Research Paper
Signal Transduction - genetics
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Summary:In this study, microRNA (miRNA) profiles in postovulatory aging mouse oocytes were analyzed by microarray screening and RT-qPCR. Hierarchical cluster analysis on the microarray data and KEGG pathway enrichment analysis on the mRNAs targeted by differentially expressed (DE) miRNAs between two adjacent egg-ages suggest that while only a mild alteration in miRNA expression occurred from 13 to 18 h, a great change took place from 18 to 24 h post hCG injection. Theoretical exploration on functions of the predicted target genes suggest that KEGG pathways enriched by 13-18 h DE miRNAs are correlated with early events of oocyte aging while pathways most enriched by 18-24 h or 24-30 h DE miRNAs are correlated with the late symptoms of aged oocytes. Experimental verification on functions of the key proteins predicted by the KEGG analysis and injection of miR-98 mimics or inhibitors further confirmed that miRNAs played stimulatory/inhibitory roles in postovulatory oocyte aging. In conclusion, marked changes in miRNA expression are associated with significant alterations in function and morphology of postovulatory aging oocytes.
ISSN:1945-4589
1945-4589
DOI:10.18632/aging.101219