Energy-linked sulfate uptake by corn mitochondria via the phosphate transporter

Corn shoot mitochondria possess an energy-linked transport system for sulfate uptake as demonstrated by osmotic swelling and [35S]SO4 2- accumulation. Maximum uptake is secured in the presence of Mg2+ and oligomycin with sucrose for osmotic support. Neither phosphate nor dicarboxylate anions are req...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1979-04, Vol.63 (4), p.635-638
Main Authors: Abou-Khalil, Samir, Hanson, John B.
Format: Article
Language:English
Subjects:
Anions
Corn
Dicarboxylic acid transporters
Liver mitochondria
Mitochondria
Oligomycins
Phosphate transport proteins
Phosphates
Sulfates
Swelling
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Summary:Corn shoot mitochondria possess an energy-linked transport system for sulfate uptake as demonstrated by osmotic swelling and [35S]SO4 2- accumulation. Maximum uptake is secured in the presence of Mg2+ and oligomycin with sucrose for osmotic support. Neither phosphate nor dicarboxylate anions are required. When added simultaneously, millimolar concentrations of phosphate block [35S]SO4 2- uptake after the initial minute. Mersalyl, N-ethylmaleimide, and 2,4-dinitrophenol are strong inhibitors of sulfate uptake; n-butylmalonate is a weak inhibitor. These inhibitors act in the same fashion on phosphate uptake. It is concluded that sulfate uptake in the absence of phosphate is by the phosphate transporter.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.63.4.635