Gene electrotransfer: from biophysical mechanisms to in vivo applications: Part 1- Biophysical mechanisms

Electropulsation is one of the nonviral methods successfully used to deliver genes into living cells in vitro and in vivo. This approach shows promise in the field of gene and cellular therapies. The present review focuses on the processes supporting gene electrotransfer in vitro. In the first part,...

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Bibliographic Details
Published in:Biophysical reviews 2009-12, Vol.1 (4), p.177-184
Main Authors: Escoffre, Jean-Michel, Mauroy, Chloé, Portet, Thomas, Wasungu, Luc, Rosazza, Chrystelle, Gilbart, Yoann, Mallet, Laetitia, Bellard, Elisabeth, Golzio, Muriel, Rols, Marie-Pierre, Teissié, Justin
Format: Article
Language:English
Subjects:
Biochemistry
Biological and Medical Physics
Biological Techniques
Biomedical and Life Sciences
Biophysics
Cell Biology
Life Sciences
Membrane Biology
Nanotechnology
Review
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Summary:Electropulsation is one of the nonviral methods successfully used to deliver genes into living cells in vitro and in vivo. This approach shows promise in the field of gene and cellular therapies. The present review focuses on the processes supporting gene electrotransfer in vitro. In the first part, we will report the events occurring before, during, and after pulse application in the specific field of plasmid DNA electrotransfer at the cell level. A critical discussion of the present theoretical considerations about membrane electropermeabilization and the transient structures involved in the plasmid uptake follows in a second part.
ISSN:1867-2450
1867-2469
DOI:10.1007/s12551-009-0022-7