Identification of drought stress related proteins from 1Sl(1B) chromosome substitution line of wheat variety Chinese Spring

Background Wheat, one of the most important crops, has a detrimental effect on both yield and quality under drought stress. As our preliminary experiment showed that the Chinese Spring wheat- Aegilops longissima chromosome substitution line CS-1S l (1B) had a better drought tolerance than CS, the su...

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Published in:Botanical studies 2016-08, Vol.57 (1), p.20-20, Article 20
Main Authors: Zhou, Jiaxing, Ma, Chaoying, Zhen, Shoumin, Cao, Min, Zeller, Friedich J., Hsam, Sai L. K., Yan, Yueming
Format: Article
Language:English
Subjects:
Aegilops longissima
albumins
ascorbate peroxidase
biochemical pathways
Biomedical and Life Sciences
chlorophyll
chromosomes
crops
drought
drought tolerance
Ecology
filling period
genes
globulins
gluten
Life Sciences
Molecular Biology
Original
Original Article
Plant Genetics and Genomics
Plant Sciences
proline
proteome
substitution lines
water stress
wheat
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Summary:Background Wheat, one of the most important crops, has a detrimental effect on both yield and quality under drought stress. As our preliminary experiment showed that the Chinese Spring wheat- Aegilops longissima chromosome substitution line CS-1S l (1B) had a better drought tolerance than CS, the substitution line CS-1S l (1B) was used to identify drought stress related proteins by means of a comparative proteome approach in this work. Our present study aimed to explore the gene resources for drought resistance in 1S l genome. Result Our results showed that drought stress induced downregulation of relative water and chlorophyll contents and the upregulation of proline content, and further influencing grain filling shortening and significant decrease of plant height, B-type starch granule numbers, grain number and weight. In total, 25 grain albumin and globulin protein spots were found to be specifically encoded by the 1S l chromosome. In addition, 17 protein spots respected 13 unique proteins were identified by MALDI-TOF/TOF MS, which were mainly involved in adverse defense and gluten quality. Among them, ascorbate peroxidase, serpin-Z2B and alpha-amylase/trypsin inhibitor were upregulated under drought stress. These proteins play important roles in plant drought defenses through various metabolic pathways. Conclusion Our results indicate that the 1S l chromosome of Aegilops longissima has potential gene resources that could be useful for improving wheat drought resistance.
ISSN:1999-3110
1817-406X
1999-3110
DOI:10.1186/s40529-016-0134-x