Deoxyribonucleic acid polymerase from wheat embryos

A soluble DNA-dependent DNA polymerase was extracted from wheat embryos. In vitro, the incorporation of radioactive thymidine triphosphate into acid-insoluble material is dependent upon the presence of the enzyme, all four deoxyribonucleotide triphosphates, Mg2+, and a DNA template. Incorporation oc...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1974-03, Vol.53 (3), p.377-381
Main Authors: Mory, Y.Y, Chen, D, Sarid, S
Format: Article
Language:English
Subjects:
Buoyancy
Deoxyribonucleotides
DNA
Embryos
Enzymes
field crops
Materials
Neurospora
plant biochemistry
plant physiology
Radioactive decay
Radioactive materials
Reaction products
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Summary:A soluble DNA-dependent DNA polymerase was extracted from wheat embryos. In vitro, the incorporation of radioactive thymidine triphosphate into acid-insoluble material is dependent upon the presence of the enzyme, all four deoxyribonucleotide triphosphates, Mg2+, and a DNA template. Incorporation occurs on native, alkali-denatured, and strictly double-stranded DNA. The in vitro synthesized product is a polydeoxynucleotide with a chain length shorter than the template; it has the same buoyant density as wheat embryo DNA when this DNA is used as template; and it forms a double-stranded complex with the DNA template. These data suggest that the in vitro DNA synthesis catalyzed by proteins extracted from wheat embryos occurs in a semiconservative way.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.53.3.377